Data for the course "Population Genomics" at Aarhus University

Datasets, conda environments and Softwares for the course "Population Genomics" of Prof Kasper Munch.

1.  Data.tar.gz Contains the datasets and executable files for some of the softwares
2. Course_Env.packed.tar.gz Contains the conda environment used for the course. This needs to be unpacked to adjust all the prefixes. You do this in the command line by
   1. creating the folder Course_Env:  mkdir Course_Env
   2. untar the file: tar -zxf Course_Env.packed.tar.gz -C Course_Env
   3. Activate the environment: conda activate ./Course_Env
   4. Run the unpacking script (it can take quite some time to get it done): conda-unpack
3. Course_Env.unpacked.tar.gz The same environment as above, but will work only if untarred into the folder /usr/Material - so use the versione above if you are using it in another folder. This file is mostly to execute the course in our own cloud environment.
4. environment_with_args.yml The file needed to generate the conda environment. Create and activate the environment with the following commands:
   1. conda env create -f environment_with_args.yml -p ./Course_Env
   2. conda activate ./Course_Env

The data is connected to the following repository: https://github.com/hds-sandbox/Popgen_course_aarhus. The original course material from Prof Kasper Munch is at https://github.com/kaspermunch/PopulationGenomicsCourse.

Description

The participants will after the course have detailed knowledge of the methods and applications required to perform a typical population genomic study.

The participants must at the end of the course be able to:

• Identify an experimental platform relevant to a population genomic analysis.
• Apply commonly used population genomic methods.
• Explain the theory behind common population genomic methods.
• Reflect on strengths and limitations of population genomic methods.
• Interpret and analyze results of population genomic inference.
• Formulate population genetics hypotheses based on data

The course introduces key concepts in population genomics from generation of population genetic data sets to the most common population genetic analyses and association studies. The first part of the course focuses on generation of population genetic data sets. The second part introduces the most common population genetic analyses and their theoretical background. Here topics include analysis of demography, population structure, recombination and selection. The last part of the course focus on applications of population genetic data sets for association studies in relation to human health.

Curriculum

The curriculum for each week is listed below. "Coop" refers to a set of lecture notes by Graham Coop that we will use throughout the course.

Course plan

1. Course intro and overview:
   • Coop chapters 1, 2, 3, Paper: Genome Diversity Project
2. Drift and the coalescent:
   • Coop chapter 4; Paper: Platypus
   • Exercise: Read mapping and base calling
3. Recombination:
   • Lecture: Review: Recombination in eukaryotes, Review: Recombination rate estimation
   • Exercise: Phasing and recombination rate
4. Population strucure and incomplete lineage sorting:
   • Lecture: Coop chapter 6, Review: Incomplete lineage sorting
   • Exercise: Working with VCF files
5. Hidden Markov models:
   • Lecture: Durbin chapter 3, Paper: population structure
   • Exercise: Inference of population structure and admixture
6. Ancestral recombination graphs:
   • Lecture: Paper: Approximating the ARG, Paper: Tree inference
   • Exercise: ARG dashboard exercises + Inference of trees along sequence
7. Past population demography:
   • Lecture: Coop chapter 4, Paper: PSMC, revisit Paper: Tree inference
   • Exercise: Inferring historical populations
8. Direct and linked selection:
   • Lecture: Coop chapters 12, 13, revisit Paper: Tree inference
9. Admixture:
   • Lecture: Review: Admixture, Paper: Admixture inference
   • Exercise: Detecting archaic ancestry in modern humans
10. Genome-wide association study (GWAS):
    • Lecture: Coop lecture notes 99-120
    • Exercise: GWAS quality control
11. Heritability:
    • Lecture: Missing heritability and mixed models review ; Coop Lecture notes Sec. 2.2 (p23-36) + Chap. 7 (p119-142)
    • Exercise: Association testing
12. Evolution and disease:
    • Lecture: Genetic architecture review ; Article about "omnigenic" model ; Coop Lecture notes Sec. 11.0.1 (p217-221)
    • Exercise: Estimating heritability