Micro4Nano H2020-MSCA-RISE project
Published October 17, 2022 | Version v1
Journal article Open

Hierarchical Quatsome-RGD Nanoarchitectonic Surfaces for Enhanced Integrin-Mediated Cell Adhesion

Creators

  • 1. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra 08193, Spain; Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain
  • 2. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra 08193, Spain
  • 3. Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBERBBN), Madrid 28029, Spain; Unidad de Péptidos, UB, Unidad asociada al CSIC por el IQAC, Barcelona 08028, Spain
  • 4. Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain; Unidad de Péptidos, UB, Unidad asociada al CSIC por el IQAC, Barcelona 08028, Spain
  • 5. Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBERBBN), Madrid 28029, Spain; Institut de Química Avançada de Catalunya (IQAC−CSIC), Barcelona 08034, Spain
  • 6. Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBERBBN), Madrid 28029, Spain; Nanoprobes and Nanoswitches group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona 08028, Spain; Departament de Ciència dels Materials i Química Física, Universitat de Barcelona, Barcelona 08028, Spain
  • 7. Institut de Ciència de Materials de Barcelona (ICMAB-CSIC), Bellaterra 08193, Spain; Biomedical Research Networking Center on Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid 28029, Spain; Dynamic Biomimetics for Cancer Immunotherapy, Max Planck Partner Group, ICMAB-CSIC, Bellaterra 08193, Spain

Description

The synthesis and study of the tripeptide Arg-Gly-Asp (RGD), the binding site of different extracellular matrix proteins, e.g., fibronectin and vitronectin, has allowed the production of a wide range of cell adhesive surfaces. Although the surface density and spacing of the RGD peptide at the nanoscale have already shown a significant influence on cell adhesion, the impact of its hierarchical nanostructure is still rather unexplored. Accordingly, a versatile colloidal system named quatsomes, based on fluid nanovesicles formed by the self-assembling of cholesterol and surfactant molecules, has been devised as a novel template to achieve hierarchical nanostructures of the RGD peptide. To this end, RGD was anchored on the vesicle’s fluid membrane of quatsomes, and the RGD-functionalized nanovesicles were covalently anchored to planar gold surfaces, forming a state of quasi-suspension, through a long poly(ethylene glycol) (PEG) chain with a thiol termination. An underlying self-assembled monolayer (SAM) of a shorter PEG was introduced for vesicle stabilization and to avoid unspecific cell adhesion. In comparison with substrates featuring a homogeneous distribution of RGD peptides, the resulting hierarchical nanoarchitectonic dramatically enhanced cell adhesion, despite lower overall RGD molecules on the surface. The new versatile platform was thoroughly characterized using a multitechnique approach, proving its enhanced performance. These findings open new methods for the hierarchical immobilization of biomolecules on surfaces using quatsomes as a robust and novel tissue engineering strategy.

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Additional details

Funding

European Commission
TECNIOspringINDUSTRY – ACCIÓ programme to foster mobility of researchers with a focus in applied research and technology transfer 801342
European Commission
Phoenix – Pharmaceutical Open Innovation Test Bed for Enabling Nano-pharmaceutical Innovative Products 953110
European Commission
Smart-4-Fabry – Smart multifunctional GLA-nanoformulation for Fabry disease 720942
European Commission
Micro4Nano – Multifunctional nanocarriers for nonlinear microscopy: new tools for biology and medicine 101007804