Published December 21, 2022
| Version 1
Technical note
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Purification of SLC12A5 from Expi293FTM Cells
Authors/Creators
- 1. Centre for Medicines Discovery, University of Oxford. Oxford, UK
Description
This protocol is used to purify the SLC transporter expressed in Expi293FTM Cells using detergent solubilization followed by a series of different chromatographic methods. Depending on the nature of the expression construct and experimental requirements, the GFP tag can be removed using a proteolytic reaction.
Files
SP0017-6_SLC12A5 Purification.pdf
Files
(130.8 kB)
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