Published June 6, 2022 | Version v1
Poster Open

Click Chemistry enables rapid amplification of full-length reverse transcripts for long read Next Generation Sequencing

  • 1. baseclick GmbH
  • 2. Ludwig-Maximilians-Universität München


We describe here the development of a novel click chemistry-based method for the generation and amplification of full-length cDNA libraries from total RNA, while avoiding the need for problematic template-switching (TS, standard library preparation based on the Smart-Seq2 protocol) reactions. Our method avoids random priming as well as stochastic cDNA termination, thus enabling the amplification of transcripts that were previously inaccessible by related click chemistry-based RNA sequencing techniques. 


poster SCNAC 2022-Eva.pdf

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NATURE-ETN – Nucleic Acids for Future Gene Editing, Immunotherapy and Epigenetic Sequence Modification 861381
European Commission