A semi automated in vitro model to study AMR transfer dynamics in broiler chicken caecal microbial communities.

The
continuous surveillance of antimicrobial resistance ( in farm animals is critical to safeguarding public health Alternative methods
like in vitro gut models provide valuable insight and knowledge on the AMR transmission dynamics within and between microbial
communities without using live animals Understanding these microbial dynamics is essential for developing interventions that can reduce
AMR spread among broilers and between broilers and humans In this research, we aimed to a) establish a semi automated in vitro system
able to mimic the physiological conditions of the broiler caecum (gastrointestinal organ) and maintain the main microbial communities,
and b) study the dynamics of the in vitro cultured caecal microbiota over time