RESOLUTE - Research Empowerment on Solute Carriers

Part of EU Open Research Repository
Published December 19, 2022 | Version 1
Technical note Open

Affinity Purification couple to Mass Spectrometry (AP-MS)

Creators

  • 1. CEMM - Research Center for Molecular Medicine, Vienna, AT

Description

Affinity purification mass spectrometry (AP-MS) is a high-throughput method for the isolation and identification of specific target protein interaction partners. It consists of the selective extraction of multiprotein complexes directly from cell lysates. All protein components of these complexes are then trypsin-digested and identified by nLC-MS/MS. The final output is a protein-protein interaction (PPI) data set.

Files

SP0008-8_AP-MS_v2.pdf

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Additional details

Funding

European Commission
ReSOLUTE - Research empowerment on solute carriers (ReSOLUTE) 777372