COMPARATIVE STUDY OF HEMOLYMPH PROTEIN PROFILE IN BARITELPHUSA CUNICULARIS AND PARREYSIA CORRUGATA

ABSTRACT

The freshwater molluscs and crustaceans constitute an important component of the ecosystem. The freshwater crab Baritelphusa cunicularis and Bivalve parreysia corrugata found important component of freshwater ecosystem. Proteins proved versatile macromolecules in living system and served to run biological processes. Immune system of invertebrate must rely on non-self recognized molecules to ensure defense responses against infectious pathogens that continuously threaten their survival. Attempt was made to isolate identify and characterize proteins from crab Baritelphusa cunicularis and bivalve parreysia corrugata hemolymph using SDS-PAGE. Comparatively higher numbers of proteins bands were found in crab as that of bivalve, which provides differentiating relationship among two phylum’s. Protein profiling of crab indicated proteins ranging from 13.39 to 62.73 KDa while in bivalve 17.01 to 61.98 KDa.

Keywords: Baritelphusa cunicularis, Parreysia corrugata, Hemolymph, Protein profiling, SDS-PAGE.

REFERENCES

1. Berg, J.M. Tymoczko, J.L. Stryer, L. 2002. Biochemistry 5th edition. New York: W H Freeman.
2. Wehrtmann, I.S., Célio Magalhães; Patricio Hernáez and Mantelatto, F.L. 2010. Maga Offspring production in three freshwater crab species (Brachyura: Pseudothelphusidae) from the Amazon region and Central America. Zoologia, 27(6): 965–972.
3. Nalan G, Dlua K, Yerlikayaa, P. 2003.Determination of Proximate Composition and Mineral Contents of Blue Crab (Callinectes sapidus) and Swim Crab (Portunus pelagicus) Caught off the Gulf of Antalya. Food Chem; 80: 495-498.
4. Yeo,D.C.J., Ng,P.K.L., Neil Cumberlidge, Célio Magalhães, Daniels, S.,R., and Ampos, M.R.2008. Global diversity of crabs (Crustacea: Decapoda: Brachyura) in freshwater. In E. V. Balian, C. Lévêque, H. Segers & K. Martens. "Freshwater Animal Diversity Assessment. Hydrobiologia. Developments in Hydrobiology, 198-595(1): 275–286.
5. Moore, L.E., Smith, D. M. and Loneragan, N. R. 2000. Blood refractive index and whole-body lipid content as indicators of nutritional condition for penaeid prawns (Decapoda: Penaeidae). Journal of Experimental Marine Biology and Ecology, 244(1):131–143.
6. Lorenzon, S., Giulianini, P. G., Martinis M. and Ferrero, E. A. 2007. Stress effect of different temperatures and air exposure during transport on physiological profiles in the American lobster Homarus americanus. Comparative Biochemistry and Physiology A, 147(1):94–102.
7. Terwilliger, N.B., Dangott, L, Ryan, M. 1998. Cryptocyanin, a crustacean molting protein: Evolutionary link with arthropod hemocyanins and insect hexamerins. Proc. Natl. Acad. Sci U. S. A.; 96(5): 2013-2018.
8. Depeledge, M.H., Bjerregard, P. 1989. Haemolymph Protein composition and copper levels in decapods crustaceans. Helgol. Meeresenters; 43: 207-223.
9. Sastry, A., N., and Miller, D., C., 1981. Application of biochemical and physiological responses to water quality monitoring. Biological monitoring of marine pollutants
10. F.J. Vernberg,, A. alabrese, F.P. Thurberg and W.B. Vernberg, (ed). 2010. Academic press New York. pp. 265-294.
11. Dey, A., 2008. Commercial and medicinal importance of Sundarbans. Records of Zoological Survey of India.Occasional paper 289: 1-54, ZSI, Kolkata.
12. Prabhakar, A., K., and Roy S.P. 2009. Ethnomedicinal Uses of some Shell fishes by people  of Kosi River Basin of North- Bihar. India Ethno. Med., 3(1): 1-4.
13. Janakiram, K., and Mishra, G., 2003. Homogenic and xenogenic implantation in pearl mussel surgery. Current Science, 85(6): 727-729.
14. Laemmli, U.K., 1970. Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature; 227: 680–685.
15. Florkin, M., 1960. Blood chemistry. In T.H. Waterman (ed.) Physiology of crustacean,
16. Academic press, New York, 1: 141-159.
17. Engle, R., L., and Woods, K., R., 1960. Comparative Biochemistry and embryology. F.W. Putnam(ed.) In ‘Plasma proteins’ Academic Press, New York, pp. 2: 184-206.
18. Gibert, J., 1972. Synthese bibliographique des recherches electrophoretique sur les proteins des crustacean. Ann. Biol., 11: 305-327.
19. Akbar, Z, Qasim, R., Siddiqui, P., J., A., 1988. Seasonal variation in biochemical composition of edible crab, Portunus pelagicus (Linneaus). J. Islamic Acade. Sci; 1(2): 127-133.
20. Soundarapandian, P., Ananthan, G., 2008. Effect of unilateral eyestalk ablation and diets on the biochemical composition of commercially important juveniles of Macrobrachium malcomsonii (H. Milne Edwards). Intern. J. Zool. Resear; 4(2): 106-112.
21. Sudhakar, M., Manivannan, K., Soundrapandian, P. 2009. Nutritive value of hard and soft shell crabs of Portunus sanguinolentus (Herbst). Inter. J. Ani. and Veterin. Adv; 1(2): 44-48.
22. Edward, N.J., T.A. Arroll and Fan, Z. 1996. Specificity some physicochemical characteristics of antibacterial activity from blue crab Callinectes sapidus. Fish Shellfish Immunol. vol 6, pp 403-412.
23. Hoq, M., .I. M.U. Seraj and S. Chowdhury. 2003. Isdolation and characterization of ntibacterial peptides from the muc-crab Scylla serrata. Pakisthan Journal of Biological science. vol 6, no 11, pp 1345 – 1353.
24. Ravichandran, S., K. Sivasubramaninan and R.M. Anbuchezhian. 2010. Antimicrobial activity from the haemolymph of the crab Ocypode macrocera. World Appl. Sci. J. vol 11, no 5, pp 578-581.
25. Samuthirapandian Ravichandran, Singaram Jeyalakshmi, Sathasivam Sudha and Ramasamy Anbuchezhian. 2010. Antimicrobial peptides from the haemolymph of the prawn Macrobrachium rosenbergii. Bangladesh J. Pharmacol. vol 5, pp 62-67.
26. Pan, J.Y., Y.L. Zhang and X.X. Peng. 2008. Dodecamer is required for agglutination of Litopenaeus vannamei hemocyanin with bacterial cells and red blood Cells. Mar. Biotech. vol 301, pp 39-54.
27. Maguire, G., B., and Fielder, D., R., 1975. Disc electrophoresis of the hemolymph proteins of some portunid crabs (Decapoda-portunidae) – I effects of storage. Comp. Biochem. Physiol., 52: 39-42.
28. Kannupandi, T., and Paul, Pandian, A.,L.,1975. Studies on the blood and muscle proteins of crabs of Porto-novo. Bull Dept. Mar. Sci Univ. Cochin, 7(3): 609-622.
29. Chan, S., M., S., Rankin, M., and Larry, I., Keeley, 1988. Characterization of the molt stages in Penaeus vannamei: Setogenesis and hemolymph levels of total protein, ecdysteroids and glucose. Biol. Bull., 175: 185-192.
30. Scheer, B.T. 1960. Aspects of the intermoult cycle in natantians Comp. Biochem. Physiol., 1: 3-18.