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Published October 10, 2022 | Version v1
Dataset Open

King Colobus and Western Red Colobus of Taï National Park - microsatellite dataset

  • 1. Centre for Research in Anthropology (CRIA), Av. Forças Armadas, Edifício ISCTE, sala 2w2, 1649-026 Lisboa, Portugal
  • 2. Instituto Gulbenkian de Ciência, Rua da Quinta Grande, 6, 2780-156 Oeiras, Portugal
  • 3. Senciência, Lda., Palácio Baldaya - CoWork Baldaya, Estrada de Benfica 701 - 1500-087 Lisboa
  • 4. Organisms and Environment Division, School of Biosciences, Cardiff University, Sir Martin Evans Building, Museum Avenue, Room C/5.15, Cardiff, Wales, UK CF10 3AX
  • 5. Epidemiology of Highly Pathogenic Microorganisms, Robert Koch Institute, Berlin, Germany
  • 6. Department of Human Behavior, Ecology and Culture, Max Planck Institute for Evolutionary Anthropology, Deutscher Platz 6, 04103 Leipzig, Germany
  • 7. cE3c - Centre for Ecology, Evolution and Environmental Changes & CHANGE - Global Change and Sustainability Institute, Departamento de Biologia Animal, Faculdade de Ciências, Universidade de Lisboa, 1749-016 Lisboa, Portugal
  • 8. Centre for Research in Anthropology (CRIA), Av. Forças Armadas, Edifício ISCTE, 1649-026 Lisboa, Portugal
  • 9. University of Texas at San Antonio (UTSA), Department of Anthropology, College of Liberal and Fine Arts, San Antonio TX 78249-1644
  • 10. CIBIO, Centro de Investigação em Biodiversidade e Recursos Genéticos, InBIO Laboratório Associado, Campus de Vairão, Universidade do Porto, 4485-661 Vairão, Portugal
  • 11. Laboratoire Évolution & Diversité Biologique (EDB UMR 5174), Université de Toulouse Midi-Pyrénées, CNRS, IRD, UPS. 118 route de Narbonne, Bat 4R1, 31062 Toulouse cedex 9, France

Description

Abstract:

We compared the genetic patterns - genetic diversity, population structure, and demographic history - of two African colobine species - King Colobus (Colobus polykomos) and Western Red Colobus (Piliocolobus badius) - in Taï National Park (TNP), Ivory Coast, and Cantanhez Forests National Park (CFNP), Guinea-Bissau, using a dataset of microsatellite loci. While TNP is the largest and best preserved forest of West Africa, CFNP is highly fragmented. Thus, these two forests provide the opportunity of studying the impact of forest status on the maintenance of these species' evolutionary potential.

CFNP colobines showed very low genetic diversity, while the populations in TNP still maintain high levels of genetic diversity. A strong signal of population decline was and we found no clear signal of population decrease in Western red colobus and a limited decrease in King colobus. These results suggest larger and historically more stable populations in TNP compared to CFNP. We cannot exclude the possibility that the demographic effects resulting from the recent increase of bushmeat hunting are not yet detectable in TNP using genetic data. Nevertheless, the fact that the TNP colobus populations are highly genetically diverse and maintain large effective population sizes suggests that well-preserved forests are crucial for the maintenance of populations, species and probably for the evolutionary potential in colobines.

 

Methods:

Blood-derived DNA samples of eight King Colobus (Colobus polykomos) and 20 Western Red Colobus (Piliocolobus badius) were genotyped for eleven and ten, respectively, human-derived nuclear microsatellite loci. Loci were amplified by Polymerase Chain Reaction (PCR), following the protocol optimised by Minhós et al. (2013). PCR products were analysed using an ABI 3130XL Automatic Sequencer at the Genomics Unit of Instituto Gulbenkian de Ciência, Portugal. Alleles were scored using GeneMapper® Software version 4.1 (Applied Biosystems). Each locus was genotyped up to three times per sample and two independent observers scored the genotypes. Genotypes were considered as heterozygous after each allele was observed in at least two independent PCR reactions. This dataset presents the consensus genotypes obtained for each individual at each locus. No GPS data are available for this dataset, although all individuals are known to come from Taï National Park, Ivory Coast.

 

Usage notes:

The dataset corresponds to two CSV files - one for King Colobus (Colobus polykomos) and another for Western Red Colobus (Piliocolobus badius). The header shows microsatellite names (2 columns per microsatellite, as these are diploid individuals) and each subsequent row corresponds to an individual. The first column exhibits the codes of the individuals. Missing data is coded as 0.

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