Fig. 4. Median-joining haplotype network of Pomacea canaliculata and P. maculata sequences from Peninsular Malaysia (N=108 from 90 individuals) and the native ranges (N=105) based on 409 nucleotides of the EF1α gene. The network shows the relationship between haplotypes from different geographic regions based on sequence similarity. Unique sequences within each individual were included in the alignment (sequences for homozygotes were not doubled). Node colours represent the (A) geographic location and (B) species identity of the sequences (see legends). Each node represents a unique haplotype and node size is proportional to the haplotype frequency. Branches between nodes indicate a single nucleotide substitution unless denoted by numerical values for multiple nucleotide substitutions. Red (A) and black (B) nodes represent hypothetical ancestors or unsampled haplotypes. Two major groups are framed in grey dotted lines; P. canaliculata and P. maculata.