Dynamics of endogenous intoxication in experimental diabetes mellitus

Zaiats, Liubomyr, Fedorchenko, Yuliya, Zukow, Walery. Dynamics of endogenous intoxication in experimental diabetes mellitus. Journal of Education, Health and Sport. 2022;12(10):52-59. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2022.12.10.007

https://apcz.umk.pl/JEHS/article/view/40298

https://zenodo.org/record/7127730

The journal has had 40 points in Ministry of Education and Science of Poland parametric evaluation. Annex to the announcement of the Minister of Education and Science of December 21, 2021. No. 32343.

Has a Journal's Unique Identifier: 201159. Scientific disciplines assigned: Physical Culture Sciences (Field of Medical sciences and health sciences); Health Sciences (Field of Medical Sciences and Health Sciences).

Punkty Ministerialne z 2019 - aktualny rok 40 punktów. Załącznik do komunikatu Ministra Edukacji i Nauki z dnia 21 grudnia 2021 r. Lp. 32343. Posiada Unikatowy Identyfikator Czasopisma: 201159.

Przypisane dyscypliny naukowe: Nauki o kulturze fizycznej (Dziedzina nauk medycznych i nauk o zdrowiu); Nauki o zdrowiu (Dziedzina nauk medycznych i nauk o zdrowiu).

© The Authors 2022;

This article is published with open access at Licensee Open Journal Systems of Nicolaus Copernicus University in Torun, Poland

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Received: 01.09.2022. Revised: 02.09.2022. Accepted: 30.09.2022.

UDC:616.24+616.092.9+616.379-008.64

DYNAMICS OF ENDOGENOUS INTOXICATION IN EXPERIMENTAL DIABETES MELLITUS

Liubomyr Zaiats¹, Yuliya Fedorchenko¹, Walery Zukow²

¹ Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine patfisiology@ifnmu.edu.ua

² Nicolaus Copernicus University, Torun, Poland w.zukow@wp.pl 

Abstract

Background. Nowadays, diabetes mellitus is defined as one of the global medical and social problems and shows a growing tendency. The purpose of the study was to analyze the dynamics of endogenous intoxication in experimental diabetes mellitus. Materials and methods. The experiments were performed on 88 white male Wistar rats weighing 170-210 g. Animals were divided into three groups: 1 - intact; 2 - control; 3 - experimental with a model of diabetes mellitus, which was reproduced by intraperitoneal injection of streptozotocin by “Sigma" company (USA), diluted in 0.1 M citrate buffer with a pH of 4.5, at a rate of 60 mg/kg body weight. The control group of animals received an intraperitoneal injection with an equivalent dose of 0.1 M citrate buffer solution with a pH of 4.5.

All studies were performed under thiopental-sodium anesthesia at a rate of 60 mg/kg body weight. To determine endogenous intoxication, blood sampling of middle mass molecules (MMM) was performed 14, 28, 42 and 70 days after the streptozotocin injection. Detection of MMM fractions was performed by spectrophotometer «SF46» at the wavelength of 254 nm (MMM₂₅₄) and 280 nm (MMM₂₈₀). The STATISTICA 10 program was used for statistical processing of the obtained results. Results. The conducted biochemical studies of blood serum showed that in animals with streptozotocin-induced diabetes mellitus there was an increase in the content of both MMM fractions compared to the similar indicators of the control group of animals at all stages of the experiment: in the 14 days increased by 32,9% - MMM_254, by 12.7%- MMM₂₈₀, in the 28 days increased by 44,3 % MMM₂₅₄, by 34,0 % MMM ₂₈₀, in the 42 days increased by 62,8 % MMM_254, by 64,8 % MMM₂₈₀, in 70 days increased by 76,1 % MMM₂₅₄, by 81,1% MMM₂₈₀. Conclusions. Streptozotocin-induced diabetes mellitus is accompanied by an increase in the level of endogenous intoxication which is manifested by an increase of both MMM fractions content in serum.

The severity of endogenous intoxication depends on the duration of the endogenous factor.

Keywords: Streptozotocin-induced diabetes, middle mass molecules.