Massaria racemosae Samarakoon & Liu & Karunarathna & Mukjang & Cheewangkoon 2022, sp. nov.

Massaria racemosae Samarak., sp. nov. (FIGURE 3)

MycoBank: MB843666; Facesoffungi number: FoF 10808

Etymology—The specific epithet refers to the species name of the host Ficus racemosa.

Holotype — MFLU 19-2135

Saprobic on a dead branch. Sexual morph: Ascomata 380–450 μm diam. × 290–320 μm high (x = 420 × 307 μm, n = 10), immersed in bark with erumpent neck, visible as black dots on the host surface, solitary, scattered or sometimes gregarious, subglobose, flat base, coriaceous, brown to dark brown, with centrally opening ostiole. Ostiole centrally located, filled with periphyses and hyaline cells. Peridium 18–28 μm (x = 24 μm, n = 12) wide at the base, 42–70 μm (x = 55.5 μm, n = 8) wide around the ostiole, outer layer comprising reddish to dark brown, fused with host tissues, thin-walled cells of textura angularis, inner layer composed of hyaline, loosen, cells of textura angularis. Hamathecium 1.4–3 μm (x = 2.1 μm, n = 15) wide, composed of numerous, dense, long, filamentous, branched, septate, round to blunt apex, trabeculate pseudoparaphyses, embedded in a gelatinous matrix. Asci 175–260 × 22–42 μm (x = 215 × 32 μm, n = 25), 8-spored, bitunicate, fissitunicate, cylindrical or oblong, with a short pedicel, apically flattened. Ascospores 45–56 × 11–16 μm (x = 50 × 14 μm, n = 35), overlapping 2-seriate, fusoid to ellipsoid, hyaline, many guttules when immature, 3-septate when mature, deteriorated dark brown when over mature, lumped at the septum, narrowly rounded to nearly acute at the ends, rough-walled, prominent, large rhomboid guttule in each cell, surrounded by a 9–28 μm (x = 15 μm, n = 10) wide, distinct mucilaginous sheath. Asexual morph: Undetermined.

Material examined— THAILAND, Nan Province, Pua District, on a dead branch of Ficus racemosa (Moraceae) attached to the host, 29 January 2019, MC. Samarakoon, SAMC215 (MFLU 19-2135, holotype; HKAS 106992, isotype). Additional sequence ITS: ON191541 (MFLU 19-2135).

Notes: Massaria racemosae morphologically fits into the generic concept of Massaria as above-mentioned and differs from M. broussonetiae by having sub-globose, flat base ascomata, lack of green algae-like structures, and hyaline mature ascospores. Massaria gigantispora has more frequently grouped ascomata, a distinct rosy ostiole, larger asci (300–350 × 71–83 μm vs. 175–260 × 22–42 μm), and ascospores (89–112 × 25–28 μm vs. 45–56 × 11–16 μm) compared to M. racemosae. The LSU sequence of M. racemosae is similar to that of M. parva WU 30553 (844/850; 99%), M. lantanae CBS 125592 (840/851; 99%), and M. anomia WU 30503 (840/851; 99%), while SSU is similar to that of M. lantanae CBS 125592 (786/794; 99%), M. anomia WU 30509 (786/794; 99%), and M. ulmi WU 30566 (782/794; 98%). The RPB2 sequence of M. racemosae is similar to that of M. anomia CBS 591.78 (901/1032; 87%), M. inquinans WU 30527 (899/1039; 87%), M. vomitoria WU 30606 (897/1038; 86%), and M. platanoidea WU 30554 (898/1039; 86%). Combined gene phylogenies also showed M. racemosae as a distinct taxon, thus we introduce it here as a new species.