Published August 8, 2022 | Version condensate
Software Open

Shovamaharana/Analyse_Cytoplasmic: nuclear_condensates

Authors/Creators

Description

This is a Matlab Code to analyse nuclear condensate images for enrichment of respective markers. We start here with the best focussed images of the condesates and the respective images in the dapi channel. We keep all the channel files in one folder. The channel names are usually separated by '_c001.tif', '_c002.tif' or 'c:1-3.tif', 'c:2-3.tif' etc. Each filed of view opens one after another. We use the dapi channel to automatically detect the nucleus. We separate the nucleus and look for intranuclear condensates. We use a 12 pixel rim around the nucleus to calculate cytoplasmic intensity. The results are compiled in variable-"all_result1", which can be copied into an excel.

Full Changelog: https://github.com/Shovamaharana/Analyse_Cytoplasmic/commits/condensate

Files

Shovamaharana/Analyse_Cytoplasmic-condensate.zip

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