Published July 20, 2022
| Version v1
Dataset
Open
Volumetric imaging of fluorescently labeled BPAE cells
Creators
- 1. Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico.
Description
Using the Nanoimager-S microscope (ONI, Oxford Nanoimaging) with a sCMOS sensor (Hamamatsu, ORCA-Flash4.0 V2), FluoCells™ Prepared Slide #1 (Thermo, #F36924) were imaged with a 100X, 1.4 NA, oil-immersion objective (Olympus). DAPI, Alexa-488 and MitoTracker™ Red excitation was delivered by 405 nm, 473 nm and 561 nm lasers, respectively. Light was collected while using two emission filters (1: 525/50; 2: Band 1 575-616.5) and a Channel Splitter dichroic 561 LP. A 3D Z-stack of the sample was acquired for each channel. 22 frames were generated, each separated 50 nm from each other in Z.
Files
Fluorescently labeled BPAE cells.tif
Files
(38.7 MB)
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Additional details
Related works
- Is published in
- Software documentation: 10.5281/zenodo.6836636 (DOI)
- Preprint: 10.21203/rs.3.rs-984659/v1 (DOI)
- Preprint: 10.1101/2021.10.17.464398 (DOI)