Published July 19, 2022 | Version v1
Dataset Open

Correlative 3D SBFSEM data from: Intermittent bulk release of human cytomegalovirus

Description

Human Cytomegalovirus (HCMV) can infect a variety of cell types by using virions of varying glycoprotein compositions. It is still unclear how this diversity is generated, but spatio-temporally separated envelopment and egress pathways might play a role. So far, one egress pathway has been described in which HCMV particles are individually enveloped into small vesicles and are subsequently exocytosed continuously. However, some studies have also found enveloped virus particles inside multivesicular structures but could not link them to productive egress or degradation pathways.
We used a novel 3D-CLEM workflow allowing us to investigate these structures in HCMV morphogenesis and egress at high spatio-temporal resolution. We found that multiple envelopment events occurred at individual vesicles leading to multiviral bodies (MViBs), which subsequently traversed the cytoplasm to release virions as intermittent bulk pulses at the plasma membrane to form extracellular virus accumulations (EVAs). Our data support the existence of a novel bona fide HCMV egress pathway, which opens the gate to evaluate divergent egress pathways in generating virion diversity.

Notes

The data are in the proprietary dm4 format as saved by the acquisition software. However, they can be easily opened and converted in Fiji/ImageJ with the Bioformats importer. We recommend alignment of the stack and binning of 2x2 for working with the data.

Funding provided by: Wellcome Trust
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100010269
Award Number: 209250/Z/17/Z

Funding provided by: Deutsche Forschungsgemeinschaft
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659
Award Number: 390874280

Funding provided by: Deutsche Forschungsgemeinschaft
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659
Award Number: 413831413

Funding provided by: Studienstiftung des Deutschen Volkes
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100004350
Award Number:

Funding provided by: Deutsche Forschungsgemeinschaft
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001659
Award Number: BO 4158/5-1

Funding provided by: Freie und Hansestadt Hamburg*
Crossref Funder Registry ID:
Award Number: LFF-FV 71-2019

Funding provided by: Leibniz-Gemeinschaft
Crossref Funder Registry ID: http://dx.doi.org/10.13039/501100001664
Award Number: W6/2018

Funding provided by: Wellcome Trust
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100010269
Award Number: 104913/Z/14/Z

Files

README.txt

Files (120.6 GB)

Name Size Download all
md5:eaf5310771d859c233aa6fc6737c101a
474.9 MB Download
md5:1a0a70fab54631393df5262ebf322ab3
474.9 MB Download
md5:f6db39f6f5e45137623559c0553f3c59
474.9 MB Download
md5:201d5cf90a1ab7ed275b1a8ddce044a2
474.9 MB Download
md5:a7d52869609779fbb7c5af8f4a84208d
474.9 MB Download
md5:368719db6cdf80c5c62844199d44ae5d
474.9 MB Download
md5:e5a21b66860fea474eaf59a5b0e9c479
474.9 MB Download
md5:2dafaabe6261d3b8bbdc85cb76adbcbe
474.9 MB Download
md5:28611aca83619a4d3c3e7721db1685c6
474.9 MB Download
md5:9ebb06ac51763ba7a0a9aef86b360772
474.9 MB Download
md5:684681db847e29bd882d881ff0a0d209
474.9 MB Download
md5:81ba47b0374a7f3740318015c4871cee
474.9 MB Download
md5:d83824487a21ebc330470a50c2e997a6
474.9 MB Download
md5:b8347fdb1c412361370db45fe82d700c
474.9 MB Download
md5:f2651a0390b1625c333fc820cc31125f
474.9 MB Download
md5:c91fc6f39190238aae8508d4b0b71a4a
474.9 MB Download
md5:c8a49de3509c33a7ed9e719add71ca5f
474.9 MB Download
md5:abf36e3701909de5fcde0808f55cf710
474.9 MB Download
md5:cc1e16cd66fb69394c3bd4b340ce067e
474.9 MB Download
md5:fc813effe8efd9519880ad9d33d71e3c
474.9 MB Download
md5:3ed0cb2edff41746fd3eda96977755d2
474.9 MB Download
md5:c448bff6a5167b09e20af3dace97f05e
474.9 MB Download
md5:f2cccbface7926fcfafc76e912d322df
474.9 MB Download
md5:4365abb2b656a611ba08333377caf08a
474.9 MB Download
md5:6f4ea7a2beec5068d02276427bda08d5
474.9 MB Download
md5:e38acfa4d2ba2d85e300cfda68281fe1
474.9 MB Download
md5:1bb4d70917d059bffcacce56b6a42f64
474.9 MB Download
md5:2612b3c2dbfd0cd1dfd6df1010e544b6
474.9 MB Download
md5:e000feea1bb54cb27b3f7d28402f254a
474.9 MB Download
md5:73ac02e30f01f9f3398a16c1366e2ee3
474.9 MB Download
md5:4fef8ba193d55d7963be05ad70c1daf2
474.9 MB Download
md5:60f8087ca935e2d714406db92b5dcb8f
474.9 MB Download
md5:83db7ef64fe1697e448534ae66ce78eb
474.9 MB Download
md5:7c03680daaf43f72bb3d207692cfcdbd
474.9 MB Download
md5:88b9a3075ad366746f61ba8fa17dec1f
474.9 MB Download
md5:a8852127f5a30f131789dbc5cb8f57fd
474.9 MB Download
md5:c6871428b94805b41ff9064fbc2f93ac
474.9 MB Download
md5:9eb7a31ea4b4a46c01e62c5a949936b5
474.9 MB Download
md5:a10fbd401a03ccdc3d012f5e3189fb1d
474.9 MB Download
md5:8783039447c353c42c86e19b63691a9c
474.9 MB Download
md5:7934360ecfeb8a0e96d197659038d5b7
474.9 MB Download
md5:f913760f8405d5213da5c20181a03e4f
474.9 MB Download
md5:05d5344794a20b9555bfc129eaac0378
474.9 MB Download
md5:da2efc9b809a109caaaeeda04ec8fdb7
474.9 MB Download
md5:0e1b15a5b5e0e62e60e498ec08784da3
474.9 MB Download
md5:1336d99ad64b625d25d3b8f33b015e89
474.9 MB Download
md5:16759d20ae518e2f39ddef34e0ea5e8b
474.9 MB Download
md5:f4ad53dcf80c2e53335a14ae6fc78e07
474.9 MB Download
md5:9bb02c6018c1ee1d7f101d9523be52a7
474.9 MB Download
md5:ab8f87e80f997c788845025eb78006a5
474.9 MB Download
md5:11343cb15c67bb23fc8c7b5461eaa750
474.9 MB Download
md5:6fdf03f80056f75b8a87c007d13ffb2e
474.9 MB Download
md5:3b8b3a4a1bbaae03153be18dbdcc1a4e
474.9 MB Download
md5:f3c1381816cb93f656971b4f9194ef27
474.9 MB Download
md5:b282ada24eb0f60db64a4ded48e7f1d7
474.9 MB Download
md5:5f8935f025167ac4fe2b34bf8aa34c53
474.9 MB Download
md5:915f1ddd5620cfd6e300cbb01560e013
474.9 MB Download
md5:6fcfc8a3ae848171f5e0c3fcf76cb17c
474.9 MB Download
md5:f63e0dc7e3facfe6540d28ad456b6b46
474.9 MB Download
md5:7bf8aa497d228d7894a54f52d1648212
474.9 MB Download
md5:af85d30d5f7d6bdff5aa2d63337e402a
474.9 MB Download
md5:a7321ff3e42715ffaae76291727fa69b
474.9 MB Download
md5:7aac0fe786bd9dd1d2ca1e3a03d82407
474.9 MB Download
md5:ede4c760ec7fc271516d2b202406ebb4
474.9 MB Download
md5:5ae12cb63c06c0f4b9054c680aacf5b1
474.9 MB Download
md5:d3aefa9025deb4b4d7e3dfea2456d699
474.9 MB Download
md5:9d71aba7b9181a183792073ae4d8de06
474.9 MB Download
md5:41ea879a7c2f9e35a5fb08fed061ccc5
474.9 MB Download
md5:602a7684f16598fb1ced92f96d2fadb3
474.9 MB Download
md5:dd7e569e3237e42944045ed777d96a37
474.9 MB Download
md5:c87754d58efa617dc811901de7283fab
474.9 MB Download
md5:2a98e32389fe7e7100db00afbc3d2d30
474.9 MB Download
md5:8d418ae17c512d5d7b64680063df40b5
474.9 MB Download
md5:5c4fc6b4e0544e73a8f34d36c7b98b66
474.9 MB Download
md5:fe773f8cd558d6c175db4fcce9fdd557
474.9 MB Download
md5:a09db64fface1a08b49f866d10b76ded
474.9 MB Download
md5:a6257a213d15562db7099cf439db0653
474.9 MB Download
md5:00e47c5bc9bc7fc5ccb292d1c8ecbe4c
474.9 MB Download
md5:d1cd1e1f99c6a2502dc1d7a91d9cbcd5
474.9 MB Download
md5:e41b0181d82aa6831101a86fb60bd7f6
474.9 MB Download
md5:ce0c93d58f38f84c598543cb618efeb9
474.9 MB Download
md5:b0d5c4d00157d9a4bb79d1b7a239b12f
474.9 MB Download
md5:03ca8ca8077f5a8f11fc413eaa66e265
474.9 MB Download
md5:445ed4559c7c4e454fbef37df6bea82b
474.9 MB Download
md5:1d4d5afbb6e19422f057ff89412e458f
474.9 MB Download
md5:20e5708d133c2ce64809bb42af1417c0
474.9 MB Download
md5:e6c0e31f82de19db2e21707b70652e1b
474.9 MB Download
md5:810c2f8c7a35831ad8be84298e04324c
474.9 MB Download
md5:3d8922d157433b53e0b6820ebdecaeb1
474.9 MB Download
md5:1987a2c9315e5512bc5fa9b7095f2afa
474.9 MB Download
md5:eefeb646c950400712114059461296e4
474.9 MB Download
md5:5d0cb410f476d7419b28473dd5e86b7b
474.9 MB Download
md5:2200bd21603ad17a328238d2425d3e3f
474.9 MB Download
md5:94fbdfcb1893c3ecd8f6bc23ecfc50f0
474.9 MB Download
md5:8a573b1d5f5bf421f0bc1840ff84dded
474.9 MB Download
md5:14d40c07ee224dfaea59adfc5aa6f140
474.9 MB Download
md5:a9c844f4dce093fe43080ce927e4316e
474.9 MB Download
md5:f42be7776a473582e36aeccb7dcb3f67
474.9 MB Download
md5:9d1a2183e3077da7d13a0f1948ff5f9d
474.9 MB Download
md5:ec13fe39b10033d2e1de09bfcaca251e
474.9 MB Download
md5:7662978de284ae750a5144a14aa163c1
474.9 MB Download
md5:4716b408a491d6fc7b0a07769910f6da
474.9 MB Download
md5:427d0464ff2ce4059f398c6f0219b968
474.9 MB Download
md5:485f93c580261c5b5156d4f4f8468fb4
474.9 MB Download
md5:38d7a19309ed67816aa7d67554cd276a
474.9 MB Download
md5:15da0a6ccc36a55a914d7b89c17f537c
474.9 MB Download
md5:cc7164e121c6f7e0a75238c1d7e5c844
474.9 MB Download
md5:dd56a6aa5b3a05631464a4d87f8f10c9
474.9 MB Download
md5:a21e14cd26b3724705ad79f11f9c7d15
474.9 MB Download
md5:f7a8310dd42367abb117dbb4fda5209e
474.9 MB Download
md5:f0d4c08d22af4dc787ffd61fde8c545c
474.9 MB Download
md5:c6163330f2d7aa50ec9d43b5fcb0073c
474.9 MB Download
md5:6430b43c0a706d512e9ebf24d37d9264
474.9 MB Download
md5:4a9fcabd29045256533e6fe23c9d4b26
474.9 MB Download
md5:849ab9b8b30da7df52c65dbd10771194
474.9 MB Download
md5:21233c7316db0b30b724ca52c4a393a3
474.9 MB Download
md5:64fbe800152e8c1724c1e13178bb01e8
474.9 MB Download
md5:8fe628ea62742304c7f86f809014b29f
474.9 MB Download
md5:ddb72886858153e4772753f60adb8863
474.9 MB Download
md5:f17a33e78fe3310026defc8704904684
474.9 MB Download
md5:df875eb08ed76f0b49b3e58c270a7f54
474.9 MB Download
md5:817b3014ac3e0e29acb7a1c4a7b3b5c0
474.9 MB Download
md5:1ff117e664c8cc869c9311d3e22bd6fc
474.9 MB Download
md5:ddd047b86bbff1e1a60a0f81c1ea8673
474.9 MB Download
md5:2e99798e9e841831e7027af7e0451498
474.9 MB Download
md5:ccb1d9da44cb6a350a5f3590f6556b49
474.9 MB Download
md5:256a0f73e1befe8208cb6d7427616f46
474.9 MB Download
md5:e7134c10efb2dff79d4dd09910ea3974
474.9 MB Download
md5:32baa6e7aaba4ed09d92cb9db0083833
474.9 MB Download
md5:e7a74dff4971e4f9539424ad37997f3f
474.9 MB Download
md5:cd8511e674699f5cb48e42c963e77675
474.9 MB Download
md5:078eca3a79a9db859eb971c87b69f34b
474.9 MB Download
md5:0fc6b3459cd2c91cf8e72a69f274a135
474.9 MB Download
md5:964f7e3f0bf3e3aa756b6c48403bd486
474.9 MB Download
md5:fb225422a6fe4a6ccb25235d0ada20ec
474.9 MB Download
md5:6d976b0cc2ea4dd63154aef8a847414e
474.9 MB Download
md5:051e24cae51e8eebd9b409cfc131a151
474.9 MB Download
md5:8d931fa46d7042758bb41a6db0ef8998
474.9 MB Download
md5:421482e02a70a489033e614b3bb58cc1
474.9 MB Download
md5:d099ce7557045ebbcba5525099d0eee4
474.9 MB Download
md5:7853240e806c3dd6af02d4633ff2dec3
474.9 MB Download
md5:f30650112bd8d32bbbbd2eb6a6f500d4
474.9 MB Download
md5:070789846939f4fc502d2a7bca7894ec
474.9 MB Download
md5:f1316c6c1429e4ab365081ef5fe2a7ec
474.9 MB Download
md5:e0c09bcafe509b58ef362ac6c35271ab
474.9 MB Download
md5:3f5b5815300b7dd1f662755585e384d6
474.9 MB Download
md5:b8240ebb0507daa186a6021b1ef46b55
474.9 MB Download
md5:56d60d2dc935ead6596bda1ae281c1fa
474.9 MB Download
md5:afc37bba308d430768ca078fac896715
474.9 MB Download
md5:837cd4574d21c5c95fc3e4bf7d4b8780
474.9 MB Download
md5:866cec63ad53519b0cab3e834c388e74
474.9 MB Download
md5:a92978c55879b68d15c5179a7c16b6aa
474.9 MB Download
md5:130a8721c1c0d7b6375684954407bb0f
474.9 MB Download
md5:a210c588b3cfdc8cecbcb539bb62bb1c
474.9 MB Download
md5:781c2cabcbd7bfa020cf458b17ddc75d
474.9 MB Download
md5:06c7d9c837bc22f09ef8cf5ecaecf48d
474.9 MB Download
md5:9f96f75d22bebc862b310a9c3358c680
474.9 MB Download
md5:c54e0ef3477fc91e7e6c261ef14ed85a
474.9 MB Download
md5:7df07564c2b977e99a072f1e88a1ece7
474.9 MB Download
md5:539a059f15acc48e1850c59cfe455b22
474.9 MB Download
md5:793ced510017813bafa019a1969300f0
474.9 MB Download
md5:504cb3365ecdf911a3ab9606788b8140
474.9 MB Download
md5:73aefbbe4618a94864f76a9ee84014b2
474.9 MB Download
md5:96c2ea1e1970e96492a0b5bf6e2ef250
474.9 MB Download
md5:c44e0f3d6f0dac038bb56358f27f4304
474.9 MB Download
md5:390203b4c5f1903dede6b68cb39b441e
474.9 MB Download
md5:a2794d9ff6ddbbd923b1e9c9075ab19a
474.9 MB Download
md5:f45f3cc2af701e904d049526631346e6
474.9 MB Download
md5:3cb1290d4a010763cec3cd8f42997970
474.9 MB Download
md5:d92a7a39a651f441de01400a53c95a70
474.9 MB Download
md5:9fffb3e0b67efe37d7eda74bac6327e3
474.9 MB Download
md5:c6998f9c52c66bd87f1c972e9e262a6c
474.9 MB Download
md5:9073316d8d962ed01e0ba08d156557b9
474.9 MB Download
md5:06ab21f0361790996478f11e57b05a41
474.9 MB Download
md5:5af9f23e8a3d4a03d6e1ee0da58337f3
474.9 MB Download
md5:4931f0751126831f89e378675a42d0d0
474.9 MB Download
md5:72e8d5aff05229c05ce8350dd7da03eb
474.9 MB Download
md5:1470ca45e91136edd84079af1dce55b0
474.9 MB Download
md5:4a79c34abd7eb5f818a697537788d407
474.9 MB Download
md5:3ff0656b0d6e5d61443ca07b9d8b9270
474.9 MB Download
md5:79eb306ffb3c7d15570d1d825858920f
474.9 MB Download
md5:9ad0f7c800f1ff25690f6f8d5e99c803
474.9 MB Download
md5:3297f6eb8f66c1a035caeb314cf59cdf
474.9 MB Download
md5:991d88b72fe0770377f641529c53b953
474.9 MB Download
md5:ded2e2fbb41b4aca04e3c218ccf736d0
474.9 MB Download
md5:56c86940b1fe0e0f6d095c44fa4d605e
474.9 MB Download
md5:a8f5185b58e2a15fd9d17583a72b5136
474.9 MB Download
md5:5027545c93e20724f85cdb80cc6b6022
474.9 MB Download
md5:219208f017e59ef01d0853cf419692e1
474.9 MB Download
md5:2445213b79904a5510de1377114ae037
474.9 MB Download
md5:fc803a7e3173cf6db2d6aa872fa7c330
474.9 MB Download
md5:3c2f71fc8f572ce54ff4b4d849a0ea64
474.9 MB Download
md5:3e1449761b3390ca3ef51c7398c06055
474.9 MB Download
md5:99ad5ae1af6d75530312854f03d0954d
474.9 MB Download
md5:ec96f4dbb3e7ec427b93e2f788bcccf3
474.9 MB Download
md5:c3eb5b00f62867bd400d8c5583121043
474.9 MB Download
md5:200272a9c0d4f097e4418e29c60241f6
474.9 MB Download
md5:d4abef8960c55ee3697d1e8148086180
474.9 MB Download
md5:50a1d95d7bf61dcd3bb134c6625f7943
474.9 MB Download
md5:dddfaa28a72bca9f978ae57a91310656
474.9 MB Download
md5:6e9742105eca26fb91309de7a7d243c4
474.9 MB Download
md5:5f51781602c20b7740cce479ded46b70
474.9 MB Download
md5:18eaaaddc94e9c369da93e7d045a1b68
474.9 MB Download
md5:7734595512cc31c7a56d2dbfd1378f3f
474.9 MB Download
md5:28e1555e23643ac05e38fb14beaa8c75
474.9 MB Download
md5:7b99176744519423042311ecb1946fe1
474.9 MB Download
md5:07e44a6dbd70bcb6a85e774055401040
474.9 MB Download
md5:2376bfe292dc3651918df4cc782c0b1b
474.9 MB Download
md5:c070370e1e8474bb78319b4641980666
474.9 MB Download
md5:51541dc564e14b599b433cdeacd04b51
474.9 MB Download
md5:d97f11342675d018b07f3ca307153261
474.9 MB Download
md5:29e8925c730c7ff0bf88044cae1b88f9
474.9 MB Download
md5:50046493b2d4942779f880c2429dce69
474.9 MB Download
md5:026f220ff9f7337909127d2e039b533f
474.9 MB Download
md5:318bc44fa65fc4bfce3a52d9d26af945
474.9 MB Download
md5:e9746c4de5ad360734ef477702b833bb
474.9 MB Download
md5:a12d929e7d537f22c93103a34d776a15
474.9 MB Download
md5:5d3481e7a06a7c5eb6d293be0cdb47ae
474.9 MB Download
md5:1e6e19405440c8005b60508f1823034a
474.9 MB Download
md5:cbbd4b95a3cfc4de69fabbc1409b6a75
474.9 MB Download
md5:33e7baa974290ef1b87c1547d2979211
474.9 MB Download
md5:3b62330d852a979ba8574ccd790fd88c
474.9 MB Download
md5:e436901a5ca1d344cc680ae8204d3e7a
474.9 MB Download
md5:cc7783493335c2d6fdba36653f349dd7
474.9 MB Download
md5:a0edec3b82e432d58e62bcc8095c0eef
474.9 MB Download
md5:d7b9f78336c40fa7ade84bf1dfda84d2
474.9 MB Download
md5:3c8d88c7f73e549a39bb6f9bafc38a7d
474.9 MB Download
md5:aaf0e06c8d0122a4a4a1c388aed9aa5c
474.9 MB Download
md5:0fba613cc25a7ffd29ef2b4e40119037
474.9 MB Download
md5:4928931b301dcdf68655e946e75a8c71
474.9 MB Download
md5:16614d2f07bd7c69e8fc22318d0bc9c3
474.9 MB Download
md5:63e51177e22e649d59fd8a33f1ec1fa7
474.9 MB Download
md5:34223a222b25eba467550f09134b9c75
474.9 MB Download
md5:b5543ff2a426b49ea7673ceba5ebd8b4
474.9 MB Download
md5:84e3846ae6b17bd894ded72ea95e9aa4
474.9 MB Download
md5:9bd0564d1947b9fa93af9304a837ae29
474.9 MB Download
md5:67a253388dc8e6484070c5f02064d3f9
474.9 MB Download
md5:683e5eb95194542b5044de9619efb83a
474.9 MB Download
md5:74fc4679916ee528e2120dfbe53ba26c
474.9 MB Download
md5:72a8c22ab18576290ba3c508884ce7e6
474.9 MB Download
md5:85e69a8406c4dc2010f4a46353cd0d63
474.9 MB Download
md5:412b496fa6444ee4fb96b6e1aa85179c
474.9 MB Download
md5:77602928c8af8fce1c85a8cff3768082
474.9 MB Download
md5:e65ccca55ea7abfdb529832289227539
474.9 MB Download
md5:b77baf2d038d0b79dcb901b4144df3df
474.9 MB Download
md5:2336379e49e6fac8dccee8387d653db8
474.9 MB Download
md5:b0580870304b7cbdcde7c805ea4f1ad8
474.9 MB Download
md5:e2224d4a4e382106780eb1c1a488b9bc
474.9 MB Download
md5:211d1bd941fd4a7c8be51d05819fd1a9
474.9 MB Download
md5:35d7c00d948adbcf97934a4f8271be98
474.9 MB Download
md5:bcce03692d38d0f6acb0a55c12f2e730
474.9 MB Download
md5:240408ab7e130de7e09ef152814b2d8b
474.9 MB Download
md5:125338877ee2e4abfc2fd65eeaa3a6a8
474.9 MB Download
md5:ec823cba09eaacf80deb7fdeef294d4f
474.9 MB Download
md5:d7e595d83dcbef5ae10d083922276a81
16.2 kB Preview Download

Additional details

Related works

Is supplemented by
10.5061/dryad.gtht76hpt (DOI)