Raw sequencing data for studying the colonization of soil communities after glacier retreat

Libraries were prepared following the MetaFast protocol (Taberlet et al., 2018) and sequenced using the MiSeq (Bact02 and Fung02) or HiSeq 2500 (Arth02, Coll01, Euka02, Inse01, Olig01, Sper01) Illumina platforms (Illumina, San Diego, CA, USA) with a paired-end approach (2 × 250 bp for Bact02 and Fung02, and 2 × 150 bp for the others markers) at Fasteris (SA, Geneva, Switzerland). For each marker, the sequence depth corresponded to 10,000 reads per sample. USAGE NOTES: Example for Euka02 marker: The zipped folders contains raw sequence data for seven paired-end sequenced libraries. Usually two files per library are available, corresponding to the forward and reverse reads. Example of files for one library (GWM-671): 150703_SN234_A_L001_GWM-671_R1.fastq.gz 150703_SN234_A_L001_GWM-671_R2.fastq.gz When more than two files are available for the the forward and reverse reads, more than one sequencing lane was needed to guarantee the desired sequencing depth (i.e. 10,000 sequences per sample). In this case, the raw sequences of the forward and reverse sequencing were concatenated prior to bioinformatics treatment. Example of files for one library (GWM-1497): these two files were concatenated 190503_SND405_B_L001_GWM-1497_R1.fastq.gz 190503_SND405_B_L002_GWM-1497_R1.fastq.gz these two files were concatenated 190503_SND405_B_L001_GWM-1497_R2.fastq.gz 190503_SND405_B_L002_GWM-1497_R2.fastq.gz See the complete bioinformatics pipeline provided and described in the "Methods" section of the related manuscript.