Published May 12, 2021
| Version v1
Journal article
Open
Efficient and gentle delivery of molecules into cells with different elasticity via Progressive Mechanoporation
Creators
- 1. Cell Cycle, Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany
- 2. Max Planck Institute for the Science of Light & Max-Planck-Zentrum für Physik und Medizin, 91058 Erlangen, Germany
- 3. Medical Systems Biology, Medical Faculty and University Hospital Carl Gustav Carus, TU Dresden, 01307 Dresden, Germany
- 4. Cell Cycle, Biotechnology Center, Technische Universität Dresden, 01307 Dresden, Germany & The Institute of Cancer Research, London SW7 3RP, UK
Description
Progressive Mechanoporation (PM) is a novel mechanoporation method that improves delivery efficiency of molecules into cells of different elasticity. The rational design of our system and the reliable operation and easy-assembly of the PDMS-based chip is optimized to achieve multistage cell deformation through combination of features that provide an improved and unique intracellular delivery by PM. Cas9 protein-sgRNA complex are efficiently delivered high-lighting the biological, biomedical and clinical applicability of our findings. This represents a further step towards improving mechanoporation irrespectively of cell physical properties and to widen the use of the method.
Files
Uvizl 2021 Lab Chip.pdf
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Additional details
Funding
- European Commission
- UPGRADE – Unlocking Precision Gene Therapy 825825
- European Commission
- GENSURGE – Designer recombinases for efficient and safe genome surgery 742133
- European Commission
- FLAMIN-GO – From pathobioLogy to synoviA on chip: driving rheuMatoId arthritis to the precisioN medicine GOal 953121
- European Commission
- REDOXCYCLE – The molecular interface between cell cycle and redox regulation 680042