Published April 11, 2022 | Version v3
Journal article Open

Unveiling the native morphology of extracellular vesicles from human cerebrospinal fluid by atomic force and cryogenic electron microscopy

Authors/Creators

  • 1. Advanced Materials Department, Jožef Stefan Institute, SI-1000 Ljubljana, Slovenia
  • 2. Institute of Biology and Medical Genetics, First Faculty of Medicine, Charles University, CZ-12800 Prague; Czech Republic
  • 3. Department of Medical Chemistry, Biochemistry and Clinical Chemistry, Faculty of Medicine, University of Rijeka, HR-51000 Rijeka, Croatia
  • 4. Faculty of Engineering, University of Rijeka, HR-51000 Rijeka, Croatia
  • 5. Institute of Biochemistry and Molecular Genetics, Faculty of Medicine, University of Ljubljana, SI-1000 Ljubljana, Slovenia
  • 6. Institute of Pathology, Faculty of Medicine, University of Ljubljana, SI-1000 Ljubljana, Slovenia
  • 7. Pula General Hospital, HR-52100 Pula, Croatia

Description

Table S1: Description of severe traumatic brain injury patients included in the study with Glasgow coma scale (GCS) score at admission and discharge and Glasgow outcome scale (GOS) score three months after discharge; Figure S1: Representative AFM image 10 x 10 µm applied for size distribution analysis of EV pool; Figure S2: Zeta potential of EV pool obtained by TRPS with nanopore NP400 with marked value; Figure S3: Possible artefacts obtained by cryo-TEM, which are attributed to ice contamination.

Files

Supplementary_27052022.pdf

Files (613.7 kB)

Name Size Download all
md5:9c1e2ddc97a7de7b5c9a05cce0d6e161
613.7 kB Preview Download