Diamphipnoa fresiae Sánchez 2018, sp. nov.

Diamphipnoa fresiae sp. nov.

(Figs. 1–4)

Type material. Holotype male: Chile, Región Metropolitana, Cordillera Province, Cajón del Maipo, La Obra, 27 XI 1999, coll. A. Vera (MNNC). Paratypes: 5 females, Chile, Región Metropolitana, Cordillera Province, Cajón del Maipo, El Manzano 28 XI 2004 (1), 18 XII 2005 (2), 17 VII 2006 (2), coll. A. Vera (MNNC).

Etymology. The patronym honors Dr. Fresia Rojas, National Museum of Natural History of Chile for supporting my initial studies in entomology.

Diagnosis. A small species for the genus. General coloration dark green marked with brown, male with reddish tibiae. Subgenital plate of the male emarginated, tergum X truncate, paraprocts long, epiproct slightly sigmoidal with flexed apices, short and thick, ¾ of the dorsal margin with pairs of teeth. Female subgenital plate slightly sclerotized, broadly emarginated, U-shaped; spermatheca with a long duct, rolled to the right around the capsule within segment IX, oval sperm capsule. Eggs with similar poles, polar disc flat and depressed, equatorial annulus as a fibrous band, follicular cells prominent on the margin of annulus.

Description. Adults habitus: Small for family; general color dark green marked with brown (Fig. 1F); forewings with brown spots, apex with oval spots around transversal veins; hindwings translucent, with costal margin brown; oval spots surrounding intercalary costal veins.

Holotype male: Total length 17 mm; antennae length 22 mm; cercus length 11 mm; head width 4 mm; pronotum width 4 mm; pronotum length 2 mm; forewing length 19 mm.

Female (n=5); total length 24–32 mm; antennae length 2 4–27 mm; cercus length 10–11 mm; head width 4–5 mm; pronotum width 4–5 mm; pronotum length 3–4 mm; forewing length 32–36 mm.

Male head (Fig. 1A): greenish brown, anterior ocellus small, distance between the lateral ocelli 0.5X greater than with the anterior ocellus; with dark spots around the ocelli, the region anterior to the middle ocellus and a transverse line between the eyes passing over the middle ocellus; clypeus, labrum and antennae brown; antennae with greenish-brown scape and pedicel, flagellum with>73 flagellomeres; first ¼ of the flagellum alternating dark brown and light bands; labium, maxilla and palp gray.

Thorax: Pronotum transverse, width similar to the head; medial apex of anterior margin not prominent; rounded angles, posterior angles broadly convergent towards posterior; general coloration greenish brown as in Fig.1A; prosternum with truncated anterior margin (Fig. 1D, female); mesonotum dark brown, with light middle line and metanotum light brown.

Legs: Three pairs of similar coloration, anterior femur greenish-brown with irregular depigmented spots, flexor and extensor margin with interrupted black spots; tibiae reddish, with around 6 to 7 dark bands (Fig. 1A); ventral face of femur and tibiae pale-brown; tibiae with two ventral apical spurs; femur with fine setal fringes on the flexor and extensor margin; tarsi brown, first and second tarsomeres with membranous pads developed as ventral extensions; large arolium.

Wings as in Fig. 1B; light brown and green in the base; with reduced reticulate venation; rounded apex; both pairs with three bifurcations in RP and two in M; hindwing with two bifurcations in αAA2.

Abdomen: Terga brown-greenish; tergum X in dorsal view trapezoidal, wide at base, narrowing to apex, posterior angles rounded, with a posterior-medial V-shape, sclerotized emargination with acute margin (Fig. 2D); dorsally flat (Figs. 2A, B), the medial emargination continues in a ventral long toothed plate as in the Fig. 2B. Epiproct toothed on basal ½ dorsal margin, with 17 pars of similar teeth directed anteriorly, distal part of epiproct with triangular shape in lateral view (Fig. 2B); ventral lobule large and slightly sclerotized. Paraprocts (Figs. 2E, F, G) cylindrical, slightly sclerotized; lateroventral sclerite curved up wards, with subapical and middle heavily sclerotized spine as in Fig. 2F, G; ventro-medial crown of irregular spines (Figs. 2E, F); digitiform ventro-basal lobule little sclerotized; apex with membranous lobe mid-dorsal digitiform, short and rounded (Figs. 2E, F); this lobe is invaginated at rest. Subgenital plate triangular with emarginated apex (Fig. 2C).

Female: Similar to the male but larger in body size and length of the wings; pronotum with narrow posterior angles (Fig. 1C); tibiae with 6 to 7 irregular transversal dark brown bands interspersed with greenish gray. Both pairs of wings are acuminate between ScP+RA and M; with more reticular venation and a greater number of bifurcations than male`s wing, (Fig. 1E).

Abdomen (Fig. 3): dorsal brown to greenish; tergum X triangular, flat; paraproct subrectangular, apex rounded (Fig. 3A). Subgenital plate (Fig. 3A) small, with an M-shape, formed by two parallel sclerotized plates pointing at the sides anteriorly, bilobed posterior margin with rounded and slightly prominent edges, middle emargination broadly U-shaped; the middle band and the central region sclerotized; lateral sclerite slightly sclerotized; opaque surface with transverse striae.

Bursa copulatrix as a piriform sac; dorsal sclerites scarcely sclerotized, anterior and posteriorly curved; midline partially sclerotized at the posterior end and around the spermatic pore; anterior sclerite as a scarcely sclerotized plate; lateral sclerites with lanceolate shape, its internal face with smooth transverse wrinkles; both sclerites forming a wide camera in U-shape, hardly convergent towards the previous one; sclerotized ventral plate with six to seven transverse and parallel striae. Spermatheca united in the dorsal half of the bursa; a straight duct extends through segment IX; rolls to the left around the capsule (Fig. 3C); capsule oval and longitudinally disposed; the duct is five times as long as the capsule; the capsule and the turns of the duct are located on segment IX.

Eggs: Cylindrical shape (Fig. 4F), diameter 0.58– 0.55mm, length 0.38– 0.33mm; with two similar poles, flat polar disc, depressed and limited by a wide edge (Figs. 4C, E); without collar; in equatorial view the margins converge towards the poles (Fig. 4A), the equatorial annulus with prominent margins, undulating irregularly, distorting the symmetry of the hemispheres, annulus of width similar to the sum of the margins (Figs. 4A, C).

Chorion surface with a perimeter band pattern; annulus with a fibrous band; annular margins composed of 2 to 3 rows of compact follicular cells, flat and with tiny air cells (Figs. 4B, D); above the margin the follicular cells are weakly developed and progressively more demarcated towards the edge of the polar disc, which has no impressions (Fig. 4E).

Numerous micropyles such as elevated perforations located in the peripheral wall (Fig. 4B).

Remarks. In one of female paratype the forewings has RP with 4 bifurcations, M 2–3 bifurcations, and CuA with 2–3 bifurcations.