Planned intervention: On Thursday 19/09 between 05:30-06:30 (UTC), Zenodo will be unavailable because of a scheduled upgrade in our storage cluster.
iPC
Published June 17, 2021 | Version v1
Preprint Open

The RNA Atlas expands the catalog of human non-coding RNAs

Creators

  • 1. Center for Medical Genetics, Ghent University, Ghent, Belgium
  • 2. Texas Children's Cancer Center, Baylor College of Medicine, Houston, TX, USA
  • 3. Center for Medical Genetics, Ghent University, Ghent, Belgium Cancer Research Institute Ghent (CRIG), Ghent, Belgium
  • 4. Illumina, Inc., San Diego, CA, USA
  • 5. Center for Medical Genetics, Ghent University, Ghent, Belgium Cancer Research Institute Ghent (CRIG), Ghent, Belgium VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium
  • 6. Center for Medical Genetics, Ghent University, Ghent, Belgium Cancer Research Institute Ghent (CRIG), Ghent, Belgium Data Mining and Modelling for Biomedicine Group, VIB Center for Inflammation Research, Ghent, Belgium Department of Applied Mathematics, Computer Science, and Statistics, Ghent University, Ghent, Belgium Data Intuitive, Lebbeke, Belgium
  • 7. Australian e-Health Research Centre, Commonwealth Scientific and Industrial Research Organisation, New South Wales, Sydney NSW, Australia Department of Biomedical Sciences, Macquarie University, New South Wales, Sydney NSW, Australia
  • 8. Department of Data Analysis and Mathematical Modelling, Ghent University, Ghent, Belgium
  • 9. Interdisciplinary Nanoscience Centre (iNANO), Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark
  • 10. Biogazelle, Zwijnaarde, Belgium
  • 11. Department of Diagnostic Sciences, Ghent University, Ghent, Belgium
  • 12. Department of Respiratory Medicine, Ghent University, Ghent, Belgium
  • 13. VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium Data Mining and Modelling for Biomedicine Group, VIB Center for Inflammation Research, Ghent, Belgium
  • 14. Cancer Research Institute Ghent (CRIG), Ghent, Belgium Department of Data Analysis and Mathematical Modelling, Ghent University, Ghent, Belgium
  • 15. Systems Biology Initiative, School of Biotechnology and Biomolecular Sciences, UNSW Sydney, Sydney NSW, Australia
  • 16. Adult Cancer Program, Lowy Cancer Research Centre, UNSW Sydney, Sydney NSW, Australia Prince of Wales Clinical School, UNSW Sydney, Sydney NSW, Australia
  • 17. Institute of Bioinformatics and Systems Biology, National Yang Ming Chiao Tung University, Hsinchu, Taiwan
  • 18. Department of Oncogenomics, Amsterdam UMC, University of Amsterdam, Amsterdam, The Netherlands

Description

Existing compendia of non-coding RNA (ncRNA) are incomplete, in part because they are derived almost exclusively from small and polyadenylated RNAs. Here we present a more comprehensive atlas of the human transcriptome, which includes small and polyA RNA as well as total RNA from 300 human tissues and cell lines. We report thousands of previously uncharacterized RNAs, increasing the number of documented ncRNAs by approximately 8%. To infer functional regulation by known and newly characterized ncRNAs, we exploited pre-mRNA abundance estimates from total RNA sequencing, revealing 316 microRNAs and 3,310 long non-coding RNAs with multiple lines of evidence for roles in regulating protein-coding genes and pathways. Our study both refines and expands the current catalog of human ncRNAs and their regulatory interactions. All data, analyses and results are available for download and interrogation in the R2 web portal, serving as a basis for future exploration of RNA biology and function.

Files

s41587-021-00936-1.pdf

Files (3.4 MB)

Name Size Download all
md5:800b87ba2b0617fbc92d64e6ee281579
3.4 MB Preview Download

Additional details

Related works

Is previous version of
Journal article: 10.1038/s41587-021-00996-3 (DOI)

Funding

iPC – individualizedPaediatricCure: Cloud-based virtual-patient models for precision paediatric oncology 826121
European Commission