20130515_010_M95_Ron_20

User: Elodie Ey - email: eye@igbmc.fr
Mouse: M95 (group ro-20 cage conditions study) - Sex: male - Strain: C57BL/6J - Genotype: +/+ - Treatment: none
Protocol: Male/male interaction – round cage (20 min + 8 min) - File number: 1
Upon arrival, seven-week old C57BL/6J male mice were housed in groups of four in a colony room maintained at 23±1°C on a 12:12 hour light/dark cycle, with lights on at 8:00 AM. Mice had access to food and water ad libitum. One week after their arrival, the 48 males considered as occupants were socially isolated for 3 weeks before the experiments to increase their social motivation and reduce their aggressiveness. The 12 mice used as new-comers were kept in groups of four males throughout the experiment. The experiments were conducted when animals were 11 weeks of age. The day of testing, the tested mouse was left 20 min for habituation in the round test cage (Plexiglas, diameter 50 cm (30 cm high); 100 lx; clean sawdust bedding; sound-proof chamber). After this time, an unfamiliar C57BL/6 mouse of the same sex was introduced. The two animals were allowed to freely interact for 8 min. Vocalisations were recorded with a Condenser ultrasound microphone Polaroid/CMPA, the interface UltraSoundGate 416-200, and the software Avisoft SASLab Pro Recorder from Avisoft Bioacoustics (Berlin, Germany; sampling frequency: 300 kHz; FFT-length: 1024 points; 16-bit format). Simultaneously, social interactions were videotaped continuously (high-resolution CamTech Super-Hi-Res video camera; 25 fps; starting point for synchronization: the moment the hindlegs of the new comer touched the ground).
Number of audio files for each individual: 1 (the second file is the video file of the interaction)

Experiment: cage conditions (20 min hab, round)
Date experiment: 2013-04-30
Temperature: 22°C
Light cycle: day
Microphone: Avisoft Polaroid/CMPA
Acquisition hardware: Avisoft UltraSoundGate 416-200
Acquisition software: Avisoft-Recorder USG 4.2.15
Sampling frequency: 250
Bit depth: 16

Laboratory: Institut Pasteur
25, rue du Dr Roux
75015 Paris
France