Interaction of spermine with DNA, vitamin C and bovine serum albumin in the unirradiated and gamma irradiated states
Description
Institute of Nuclear Medicine & Allied Sciences, Brig. S. K. Mazumdar Road, Delhi-11 0 054, India
E-mail: snu@inmas.org
Manuscript received 20 July 2004, revised 2 December 2005, accepted 15 December 2005
Structural deformability of spermine with radiation dose (maximum 10 Gy) has been proved. Complex formation of spermine with DNA, vitamin C and BSA took place. Calibration and radiation-induced absorption changes in spermine by ninhydrin reagent has been followed quantitatively. Interaction of vitamin C with DNA and their radiation-induced changes have been reported.Interaction of spermine with DNA in the unirradiated and gamma irradiated states in 10-3 M phosphate buffer and water have been compared. Addition of spermine and vitamin C to DNA makes DNA structure more condensed. Bovine serum albumin also binds with spermine and protects it from radiation-induced degradation.
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