Published January 9, 2022
| Version v1
Dataset
Open
Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanism
Creators
- 1. Laboratoire Avancé de Spectroscopie pour les Interactions, la Réactivité et l'Environnement (LASIRE), UMR CNRS 8516, Université de Lille, Lille, France
- 2. Thermo Fisher Scientific, Eindhoven, The Netherlands
- 3. Calixar, Lyon, France
- 4. Laboratoire de Spectrométrie de Masse BioOrganique, Université de Strasbourg, CNRS, IPHC UMR 7178, Strasbourg, France
- 5. CNRS, INSERM, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Center for Infection and Immunity of Lille, Lille, France
- 6. ERL 9002 CNRS Biologie Structurale Intégrative, UMR INSERM 1167, Université de Lille, Lille, France
- 7. Institut für Organische Chemie, Gottfried Wilhelm Leibniz Universität Hannover, Hannover, Germany
- 8. Astbury Centre for Structural Molecular Biology and the School of Molecular and Cellular Biology, University of Leeds, Leeds, United Kingdom
- 9. Bruker Switzerland AG, Fällanden, Switzerlan
Description
This dataset contains raw and processed data and analyses related to the manuscript "Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanism" by the authors. A preprint of this manuscript is available on bioRxiv (https://doi.org/10.1101/2021.11.09.467682).
Notes
The authors thank Xavier Hanoulle for useful suggestions and discussions at the start of this project and Isabelle Landrieu for her support and advice. This work was funded by the Agence Nationale de Recherche grant ANR-17-CE11-0043-02 "OPEN_BAR" to FJD and a "Projets exploratoires premier soutien (PEPS)" grant by the CNRS and the University of Lille to RS. Financial support and spectrometer access by the IR-RMN-THC FR 3050 CNRS for conducting NMR experiments at the IR-RMN platforms in Lille and Grenoble is gratefully acknowledged. EPR experiments were performed within the national facility RENARD at the University of Lille (Federation IR-RPE 3443). Support by the Centre National de la Recherche Scientifique (CNRS), Université de Strasbourg (Unistra) and the French Proteomic Infrastructure (ProFI; ANR-10-INBS-08-03) is acknowledged. The authors would also like to thank the IdeX program of the University of Strasbourg for funding the Synapt G2Si instrument.
Files
FhaC_EPRdata.zip
Additional details
Related works
- Is source of
- Journal article: 10.1101/2021.11.09.467682 (DOI)
Funding
- OPEN_BAR – Fonctionally relevant conformations of the protein transporter FhaC: probing the lateral opening of a beta barrel in a biological membrane ANR-17-CE11-0043
- Agence Nationale de la Recherche