New triterpene glycosides of Clematis montana and their cytotoxic activities

Department of Chemistry, H. N. B. Garhwal University Campus, Badshahithaul, Tehri-249 199, Uttarakhand, India

E-mail: jsjangwan@yahoo.co.in,            Fax: 91-1376-232116

Manuscript received 21 August 2007, accepted 5 December 2007

Two new triterpene glycosides designated as montanosldes 1 and 2 were isolated from methanolic extract of Clematis montana. Their structures have been elucidated as 3-O-β-D-glucopyranosyl-olcanolic acid-28-O-β-D-glucopyranosyl· (1→4)-[α-L-rhamnopyranosyl-(1→2))-β-D-xylopyranoside and 3-O-β-D-glucopyranosyl·oleanolic acid-28-O-β-D-glucopy ranosy 1-( 1→4 )-[β-D-glucopyranosyl-( 1→3)-α- L-rhamnopy ra nosy 1-( 1→2) ]-β-D-xylopyranoside, respectively by NMR and MS data. Cytotoxic activity of the compounds were evaluated against various squamous carcinoma cells (HSC-2) and human gingival fibroblast cells (HGF). Both of the compounds showed potent cytotoxic activity against the test cells.