A rapid and validated RP-HPLC method for estimation of glimepiride in solid dosage form

R & D Division, Dey's Medical Stores (Mfg.} Ltd., 62, Bondel Road, Kolkata-700 019, India

E-mail : akbera2009@gmail.com Fax : 91-33-22876332

Manuscript received 23 July 2009. revised 9 February 2010, accepted 25 February 2010

A simple, accurate, rapid, precise and economic reverse phase high performance liquid chromatographic method was developed for estimation of glimepiride In tablet dosage form using C18 (5 µm, 250 mm x 4.6 mm) column. The mobile phase was a mixture of phosphate buffer (pH = 7.0 ± 0.1) and acetonitrile In the ratio 40 : 60 v/v, The detection wavelength was 228 nm. The retention time was 1.9 min at a Dow rate of 1 ml/min. The validation of the proposed method was carried out according to USP and ICH guidelines. The method was found to be linear (r² = 0.9999), precise (%RSD ≤ 2), accurate (mean percentage recovery yields = 99.31) and selective. Due to its simplicity, accuracy and economic value, the proposed method can be used for routine quality control analysis of solid dosage forms of glimepiride.