Published November 29, 2021
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SOP for metabolite profiling of seminal plasma via GC Orbitrap
Creators
- 1. RECETOX, Faculty of Science, Masaryk University, Czech Republic
Description
Seminal plasma chemical profiles provide distinct and metabolic phenotypes suited for
fertility study monitoring. Profiling via GC-MS is favourable as it provides robust measures
across many core primary metabolic pathways; including amino acids, TCA metabolites,
carbohydrates and some lipids (e.g. steroids & fatty acid). A protocol for simple preparation
of seminal plasma samples intended to undergo GC-MS analysis is described and enables
large-scale semi-quantitative profiling.
Notes
Research Infrastructure RECETOX RI (No LM2018121) financed by the Ministry of Education, Youth and Sports, and Operational Programme Research, Development and Innovation - project CETOCOEN EXCELLENCE (No CZ.02.1.01/0.0/0.0/17_043/0009632).
Files
SOP_Seminal plasma_Metabolites_KC_version 1.pdf
Files
(639.7 kB)
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