Development and validation of new RP-HPLC method for the estimation of Amlodipine besylate and Bisoprolol fumarate in bulk and tablet dosage form

Yash Institute of Pharmacy, Aurangabad-431 134, Maharashtra, India

E-mail : vandana2609@gmail.com

Manuscript received online 18 May 2013, revised 11 June 2013, accepted 11 June 2013

New HPLC method has been developed for determination of Amlodipine besylate and Bisoprolol fumarate in their binary mixture and tablets as a part of routine control of combined formulations using RP-C18 column. The method was validated to meet official requirements including selectivity, stability, linearity, precision a nd accuracy. Chromatography was carried out using a Sil C-18 HS column, a mixture containing methanol, acetonitrile and 50 Mm potassium dihydrogen phosphate buffer of pH 3.0 in the ratio of 25 : 30 : 45 % v/v/v with UV detection at 267 nm. The method was proved to complying with all official guidelines. Therefore, it is suitable for determination of Amlodipine besylate and Bisoprolol fumarate in their binary mixtures for different analytical and pharmaceutical purposes.