Description of Amycolatopsis marina sp. nov.

Amycolatopsis marina (ma.ri'na. L. fem. adj. marina of the sea, marine).

Cells are Gram-positive, aerobic and non-motile and produce white aerial mycelium sparsely on ISP 2 agar medium. The branched yellow to yellow–brown substrate mycelium fragments into rod-like elements. No diffusion pigments are produced on any of the media tested. Catalase-positive and oxidase-negative. Negative for urease and negative for reduction of nitrate to nitrite. H 2 S is not produced. Growth occurs between pH 6.0 and 9.0, between 10 and 45 °C and between 0.5 and 12 % NaCl, but not above 45 °C or above 12 % NaCl. The optimum growth pH, temperature and NaCl are 7.0–8.0, 28 °C and 5 % NaCl. Resistant to rifampicin, amikacin, carbenicillin, tobramycin, kanamycin, clarithromycin and penicillin G (each at 30 µg), but sensitive to gentamicin, novobiocin, streptomycin, doxycycline, acetylspiramycin, carbenicillin, midecamycin, minocycline, cephalothin and chloramphenicol. (+)-D-Fructose, (+)-D-galactose, (+)-cellobiose, myo -inositol, (+)-L-rhamnose salicin, (+)-maltose, (+)-D-mannitol and (+)-trehalose are utilized as carbon sources, but sorbitol, (+)-D-lactose, dextrin, arabinose, (+)-raffinose and (‾)-sucrose are not. Decomposes gelatin, hypoxanthine, xanthine, allantoin and starch, but not L-tyrosine or casein. Cell-wall hydrolysates contain meso -diaminopimelic acid, arabinose and galactose. MK- 9 (H 4) (79 %) is the predominant menaquinone; MK- 8 (H 4) (13 %) is also present. The phospholipids comprise diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol and phosphatidylinositol mannoside. The major cellular fatty acids are iso-C 16: 0 and iso-C 16: 0 2 - OH. The DNA G+C content of the type strain is 70.1 mol%.

The type strain, Ms 392 A T (= CGMCC 4.3568 T = NBRC 104263 T), was isolated from an ocean-sediment sample collected in the South China Sea.