Description of Klebsiella spallanzanii sp. nov. and of Klebsiella pasteurii sp. nov.
Creators
- 1. Fondazione IRCCS Policlinico San Matteo, Unità Operativa Complessa Microbiologia e Virologia, Pavia, Italy; Scuola di Specializzazione in Microbiologia e Virologia, Università degli Studi di Pavia, Pavia, Italy,
- 2. Biodiversity and Epidemiology of Bacterial Pathogens, Institut Pasteur, Paris, France
- 3. Fondazione IRCCS Policlinico San Matteo, Unità Operativa Complessa Microbiologia e Virologia, Pavia, Italy,
- 4. Department of Biology and Biochemistry, The Milner Centre for Evolution, University of Bath, Bath, United Kingdom
- 5. Infection Genomics, Wellcome Sanger Institute, Cambridge, United Kingdom; Department of Biostatistics, University of Oslo, Oslo, Norway
- 6. Center of Infectious Diseases, West China Hospital, Sichuan University, Chengdu, China
- 7. Department of Biosciences, University of Milan, Milan, Italy; Pediatric Clinical Research Center "Romeo ed Enrica Invernizzi", University of Milan, Milan, Italy
- 8. Dipartimento di Biologia e Biotecnologie "L. Spallanzani", Università di Pavia, Pavia, Italy
- 9. Department of Mathematics and Statistics, Helsinki Institute for Information Technology HIIT, University of Helsinki, Helsinki, Finland
Description
Klebsiella oxytoca causes opportunistic human infections and post-antibiotic
haemorrhagic diarrhea. This Enterobacteriaceae species is genetically heterogeneous
and is currently subdivided into seven phylogroups (Ko1 to Ko4 and Ko6 to Ko8).
Here we investigated the taxonomic status of phylogroups Ko3 and Ko4. Genomic
sequence-based phylogenetic analyses demonstrate that Ko3 and Ko4 formed well defined
sequence clusters related to, but distinct from, Klebsiella michiganensis (Ko1),
K. oxytoca (Ko2), K. huaxiensis (Ko8), and K. grimontii (Ko6). The average nucleotide
identity (ANI) of Ko3 and Ko4 were 90.7% with K. huaxiensis and 95.5% with
K. grimontii, respectively. In addition, three strains of K. huaxiensis, a species so far
described based on a single strain from a urinary tract infection patient in China, were
isolated from cattle and human feces. Biochemical and MALDI-ToF mass spectrometry
analysis allowed differentiating Ko3, Ko4, and Ko8 from the other K. oxytoca species.
Based on these results, we propose the names Klebsiella spallanzanii for the Ko3
phylogroup, with SPARK_775_C1T (CIP 111695T and DSM 109531T) as type strain, and
Klebsiella pasteurii for Ko4, with SPARK_836_C1T (CIP 111696T and DSM 109530T) as
type strain. Strains of K. spallanzanii were isolated from human urine, cow feces, and
farm surfaces, while strains of K. pasteurii were found in fecal carriage from humans,
cows, and turtles.
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