GC-MS ANALYSIS OF BIOACTIVE COMPOUNDS USING METHANOLIC EXTRACTS OF NARINGI CRENULATA (ROXB.) NICOLSON LEAVES.

Herbs are very important for a researcher as most of the pharmaceutical companies depend on these plants for synthesizing various novel compounds. The crude plant extracts which is usually a complex mixture of various bioactive compounds including its secondary metabolites forms the effective  herbal medicines. The aim of the current study is to determine the bioactive compounds from the methanolic extracts  of the leaves of  Naringi crenulata (Roxb.) Nicolson (MENC) using GC-MS. This  analysis was done by standard protocol using the equipment  Thermo GC-Trace Ultra Version: 5.0, Thermo MS DSQ II, the extract was matched with the National Institute of Standards and Technology (NIST) library. 26 components from leaves of the above said plant were identified. The GC-MS analysis disclosed  the presence of various  important phytoconstituents like  carotene, lycopene, lycoxanthin, astaxanthin and vitamin D which is responsible for its antioxidant property. It was also observed that the presence of  hexadecanoic acid revealed its anti-inflammatory activity, followed by stigmast has an anti- diabetic activity and cycloheptasiloxane has an emollient activity. These findings support the traditional use of Naringi crenulata in various disorders. Further studies are needed to isolate active principle of the extract as well as to elucidate their exact mechanism of action in various disorders.

Keywords: Naringi crenulata, bioactive compounds, GC-MS analysis, MENC.