UV-VIS Spectra Activated Droplet Sorting for Label-Free Chemical Identification and Collection in Droplets

Data underlying the figures in the publication “UV-VIS Spectra Activated Droplet Sorting for Label-Free Chemical Identification and Collection in Droplets”, published in Analytical Chemistry, 2021.

Table of contents:

1. Fig2_BSA_calibration_2000us; Excel file containing the BSA UV-VIS spectra corresponding to Figure 2. It demonstrates the calibration of the UV-VIS in-droplet measurement. Spectroscopy settings: 2000 us integration time and 200 nm to 800 nm wavelength range. Samples: PBS (control), 500 uM BSA, 250 uM BSA, 100 uM BSA, 50 uM BSA, and 10 uM BSA.

2. Fig3_Data; Excel file containing the UV-VIS time-lapse data for high-throughput droplet measurements used in all subfigures of Figure 3. Fig3data.csv is a semi-colon separated csv file for UV-VIS measurements collected over ~30 minutes. Saved wavelengths include 250, 280, 300, 311, 315, 400, 500, and 600 nm. An integration time for the spectrometer of 500 us was used.

3. Fig4_C_Postsorting and Fig4_C_Presorting; Overlaid bright field & fluorescent images of droplets containing E. coli expressing gfp microcolonies before and after droplet sorting.

4. Fig4ABD_Data; Zip. archive containing the experimental data for Figure 4. Interaction of polymersome-based melanosome mimics with HaCaT cells. (A) Cell proliferation assay showing the non-toxicity of different polymersomes encapsulating dopamine and Tyrosinase (dashed purple), L-DOPA and

5. Fig5A_Combined Data; Excel file containing the UV-VIS spectra for Figure 5A. Separate sheets contain spectra for the 3 conditions: empty droplet, mutant (ergothionase activity negative) droplets, and wild type (positive) droplets. UV-VIS range from 200 nm to 1000 nm.

6. Fig5B_Ergothionase assay_wild type mutant mix; Excel file containing the UV-VIS time-lapse data for high-throughput droplet measurements used in Figure 5B.

“Fig5B_Ergothionase assay_wild type mutant mix.csv” is a semi-colon separated csv file. Wavelengths 280, 311, 400, and 600 nm are saved. An integration time of 2000 ms was used.

“Fig5B_Ergothionase assay_wild type mutant mix_settings.conf” is a configuration file for the spectrophotometer used for this measurement. It describes all spectrometer settings used in the droplet sorting process.

7. Fig5C_Combined Data; Excel file containing the 311 nm absorbance data of Figure 5C; from droplets before and after droplet sorting. The data from both conditions are separated as sheets within the file. An integration time of 2000 ms was used for these measurements.

8. SI_Absorbance_by_Geometry; Excel file containing the data used in Figure S1 to test different UV-VIS detection region geometries.