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Published August 28, 2021 | Version 1.0.0
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Hexagenia limbata early instar transcriptome assemblies

  • 1. University of New Hampshire
  • 2. Plymouth State University
  • 1. Plymouth State University
  • 2. Colby College

Description

These two transcriptomes were assembled from the same set of reads (NCBI SRA accession SRX6489924), which were sequenced from a pooled sample of approximately 100 μl of whole-body nymphs, most 1st instar and some 2nd instar.

The transcriptomes differ in their assembly methods, with CLC referring to assembly within CLC Genomics WorkBench v.6.0.4 (CLCBio, Boston, MA), while ORP refers to contig assembly via the Oyster River Protocol v2.2.6 (MacManes, 2018).

This data is part of a publication in process; the CLC contig data and original read sequencing methodology is also described in the masters thesis of coauthor Christopher Gonzalez (thesis title: "Evolutionary Developmental Biology in the Mayfly Hexagenia limbata, with a Focus on Hox Genes.")

 

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