Figs. 1–35

Ceratopogon pictus Coquillett, 1905: 60 (female; USA, Virginia). Preoccupied by C. pictus Meigen.

Hartomyia picta (Coquillett): Malloch, 1915: 341 (male; USA, Illinois; comb.).

Stilobezzia coquilletti Kieffer, 1917: 308 (new name for C. pictus Coquillett); Johannsen, 1943:781 (distrib.); Johannsen, 1952: 162 (in key); Wirth, 1953: 75 (male descr., female redesc.; distrib.); Woke, 1954: 69 (Panama; notes); Lane & Forattini, 1956: 213 (adult redesc.; Neotropical distrib.); Lane & Forattini 1961: 85 (pupa descr.; distrib.); Wirth & Grogan, 1981: 73 (adult, pupa redescr.; biology; distrib.); Borkent & Wirth, 1997: 110 (World catalog); Borkent & Spinelli, 2000: 54 (south USA catalog); Borkent & Spinelli, 2007: 30 (Neotropical catalog).

Diagnosis of Adult (Figs. 1–5, 34, 35). A yellowish species of the subgenus Stilobezzia s. str. Female flagellomeres pale except apices brown; palpus dark brown; scutum yellowish, anterior mesal margin darker, with two anterolateral and two posterolateral brown spots; wing hyaline with small brown spots over crossvein r-m, apex of R 3 and large brown spot in r 3 below apex of second radial cell; one ovoid spermatheca with short neck; gonocoxites connected by a strong bar dorsal to aedeagus; parameres stout, connected by transversal bar, each one with basal portion highly sclerotized, main portion stout with subapical, pointed projection, tip spinelike; aedeagal sclerites slightly sinuous, with blunt, hairy tip.

Description of fourth instar larva (Figs. 8–19). Head capsule (Figs. 8, 13–15) pale brown, elongate, tapering to rounded apex; chaetotaxy as in Figs.13–14; HL 0.23 mm (SEM), 0.25 mm (BCM); HW 0.15 mm (SEM), 0.13 (BCM); HR 1.35 (SEM), 1.92 (BCM); SGW 0.10 mm (SEM), 0.09 mm (BCM); SGR 1.50 (BCM). Labrum (Figs. 8, 13, 15) as long as greatest breadth; palatum (Figs. 15, 18) with three pairs of sensilla styloconica (one large, two short) on its anterior edge, pair of sensilla trichoidea immediately underneath (Figs. 18, 19); messors (Figs. 16, 18, 19) small, thin, curved, well sclerotized; well developed palatal bar (Figs. 18, 19). Maxilla (Figs. 15, 17) sclerotized, with short, slender papilla; galeolacinia with bundle of four setae; maxillary palpus cylindrical, with 3–4 papillae of different length, flaplike lobe lying alongside. Mandible (Figs. 8, 11, 15) bidentate, thin, curved, strongly sclerotized; well defined, long, apical tooth, proximal tooth small; evident fossa mandibularis on ectal surface, MDL 0.07 mm. Hypostoma (Figs. 8, 15–18) with smooth mesal elevation flanked by serrate margins, with 8–9 teeth. Epipharynx (Figs. 8–10) massive with three combs; comb 4 massive, its posterior margin irregular, with 9–10 lanceolate, subequal, mesal teeth, lateral teeth strong, pointed, largest than mesal ones; other two combs superimposed, reduced, not in position to be observed; dorsal comb sclerites with irregular posterior margin with 9–10 lanceolate teeth; each lateral arm membranous, without curtain or fringe; LAW 0.11 mm, DCW 0.07 mm. Hypopharynx (Fig. 8) robust, thick, posterior end of each arm irregular, hipopharingeal fringe no visible. Thoracic pigmentation not visible. Caudal segment wide, chaetotaxy as in Fig. 12; one pair of subequal, medium-sized, thin setae “o”; CSL 0.30 mm, CSW 0.18 mm, OD 0.08 mm.

Description of pupa (Figs. 20–33). Exuviae pale brown. Operculum (Figs. 20, 26), hexagonal, disk smooth; anteromarginal tubercle (am) (Fig. 26) strong, elongate, with stout spur-like, basal sensillum; OL 0.08 mm; OW 0.13 mm; OW/OL 1.68. Thorax surface smooth, with medial crest extending between bases of respiratory organs. Thoracic tubercles as follows: anterodorsal tubercle (ad) (Figs. 21, 31) with one mediumsized stout seta, one basal pore, wide base; dorsolateral tubercle (dl) (Figs. 24, 31) with two setae, one long, thin, hyaline, other stout, short, flattened base; 5 dorsal sensilla (d) (Fig. 21): i pore, ii with stout mediumsized seta, tringular base, iii without seta, raised base, iv small flattened base, v pore; two dorsomedial hyaline setae (dm) (Figs. 22, 31), one long, thin, triangular base, other medium-sized, flattened base. Respiratory organ (Figs. 20, 23, 30) pale brown, dark brown at tip, long, slender, nearly parallel sides, nearly straight, rounded apex; 12–14 apical pores, 4–5 basal pores opening at tubercles, length 0.38 (0.36–0.39, n=3)mm; pedicel dark brown, smooth, stout, wide base; length 0.16 (0.14–0.19, n=3) mm, P/RH 0.44 (0.41–0.48, n=3). Two ventromedian setae (vm), only insertions visible, 2 ventrolateral setae (vl), one stout, medium-sized, other pore (Figs. 20, 28). Abdominal segments integument with narrow band of sharp spinules on anterior margin, tubercles strong, spiniform, with well sclerotized apex; fourth abdominal segment tubercles (Figs. 20, 27, 32, 33) as follows: 5 dorsoposteromarginal tubercles (dpm), i–ii with minute seta, rounded base, iii–iv with thin, minute setae, conical base, v with short strong seta, conical base; 2 dorsoanterosubmarginal tubercles (dasm), i small triangular base, ii strong triangular base, i–ii with short, thin seta; 2 lateral anteromarginal tubercles (lasm), i–ii pore; 3 lateral posteromarginal tubercles (lpm), i–iii with short, strong seta, conical base; 3 ventroposteromarginal tubercle (vpm), i with strong, short seta, conical, strong, wide base, ii with thin, medium-sized seta, doble conical base, iii with short, thin seta, minute rounded base. Female anal segment (Figs. 20, 29) dorsal surface densely covered at base with posteriorly directed, pointed spicules, apicolateral process triangular, smooth, long, pointed, wide base, directed laterally, extreme tips darkish; length 0.21 (0.21–0.22, n=3) mm, width 0.16 (0.14– 0.18 n =3) mm. Male anal segment as in female, length 0.24 (0.20–0.28, n=3) mm, width 0.15 (0.14–016, n=3) mm.

Distribution. USA (Virginia, Illinois, Maryland), Mexico (Chiapas, Yucatán) to Panama, Trinidad, Brazil (Manaus, Sao Paulo)

Specimens examined. Brazil, Manaus, San Jose, 03°03´48.1”S, 59°57´46.1”W), 13-X-2005, D. Silveira Carrasco, 5 females (3 with pupal exuviae), 5 males (3 with pupal exuviae), 1 larva exuviae.

Specimens examined with SEM. Brazil, Manaus, San Jose, 13-X-2005, D. Silveira Carrasco, 2 larvae.