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Published February 2, 2019 | Version v1
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Data from: Destabilizing mutations encode nongenetic variation that drives evolutionary innovation

Authors/Creators

  • 1. University of California, San Diego
  • 2. Yale University

Description

Evolutionary innovations are often achieved by repurposing existing genes to perform new functions; however, the mechanisms enabling the transition from old to new remain controversial. We identified mutations in bacteriophage λ's host-recognition gene J that confer enhanced adsorption to λ's native receptor, LamB, and the ability to access a new receptor, OmpF. The mutations destabilize particles and cause conformational bistability of J, which yields progeny of multiple phenotypic forms, each proficient at different receptors. This work provides an example of how nongenetic protein variation can catalyze an evolutionary innovation. We propose that cases where a single genotype can manifest as multiple phenotypes may be more common than previously expected and offer a general mechanism for evolutionary innovation.

Notes

Petrie Science 2018 Fig 1B and S1
Data collected for results shown in figure 1B and S1, see notes within the file and the manuscript for further guidance
Dryad Petrie Science 2018 Fig 1B and S1.csv
Petrie Science 2018 Fig 1C and S2
Data collected for results shown in figures 1C and S2, see notes within the file and the manuscript for further guidance.
Petrie Science Fig 1C and S2.csv
Petrie Science 2018 sequence of 3-mut replicate 1
Sequence of engineered 3-mut J gene replicate 1 (see table S1 for additional guidance).
3-Mut_4-Jrev.seq
Petrie 2018 Science sequence of 3-mut replicate 2.
Sequence of engineered 3-mut J gene replicate 2 (see table S1 for additional guidance).
3-Mut_7-Jrev.seq
Petrie Science 2018 sequence of 4-mut replicate 1
Sequence of engineered 4-mut J gene replicate 1 (see table S1 for additional guidance).
4-MUT (P5_03-JRev).seq
Petrie Science 2018 sequence of 4-mut replicate 2
Sequence of engineered 4-mut J gene replicate 2 (see table S1 for additional guidance).
4-MUT (P5_04-JRev).seq
Petrie Science 2018 sequence of 5-mut replicate 1
Sequence of engineered 5-mut J gene replicate 1 (see table S1 for additional guidance).
5-MUT (Mage01_1-JRev).seq
Petrie Science 2018 sequence of 5-mut replicate 2
Sequence of engineered 5-mut J gene replicate 2 (see table S1 for additional guidance).
5-MUT (Mage01_2-JRev).seq
Petrie Science 2018 Sequence of engineered 7-mut lyso.
Sequence of engineered 7-mut lyso J gene (see table S1 for additional guidance)
31F1_1a-J_Rev.ab1-1.fa
Petrie Science 2018 Fig 2A
Data collected for results shown in figure 2A, see notes within the file and the manuscript for further guidance.
Petrie Science 2018 Fig 2B
Data collected for results shown in figure 2B, see notes within the file and the manuscript for further guidance.
Petrie Science 2018 Fig 2C
Data collected for results shown in figure 2C, see notes within the file and the manuscript for further guidance.
Petrie Science 2018 Fig 3A and 3B
Data collected for results shown in figures 3A and 3B, see notes within the file and the manuscript for further guidance.
Petrie Science 2018 Fig 3C
Data collected for results shown in figure 3C, see notes within the file and the manuscript for further guidance.
Petrie Science Fig 3D
Data collected for results shown in figure 3C, see notes within the file and the manuscript for further guidance.
Petrie Science 2018 Fig 3D.csv
Petrie Science 2018 Fig S4A and S4B
Data collected for results shown in figure S4A and S4B, see notes within the file and the manuscript for further guidance.
Petrie Science 2018 Fig S5
Data collected for results shown in figure S5, see notes within the file and the manuscript for further guidance.

Funding provided by: National Science Foundation
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000001
Award Number: DGE-1424871

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Dryad Petrie Science 2018 Fig 1B and S1.csv

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Additional details

Related works

Is cited by
10.1126/science.aar1954 (DOI)