Dryad
Published January 23, 2016 | Version v1
Dataset Open

Data from: Fat, fibre and cancer risk in African Americans and rural Africans

Creators

  • 1. University of Pittsburgh
  • 2. Imperial College London
  • 3. Wageningen University & Research
  • 4. University of Illinois at Urbana Champaign
  • 5. University of KwaZulu-Natal

Description

Rates of colon cancer are much higher in African Americans (65:100,000) than in rural South Africans (<5:100,000). The higher rates are associated with higher animal protein and fat, and lower fibre consumption, higher colonic secondary bile acids, lower colonic short-chain fatty acid quantities and higher mucosal proliferative biomarkers of cancer risk in otherwise healthy middle-aged volunteers. Here we investigate further the role of fat and fibre in this association. We performed 2-week food exchanges in subjects from the same populations, where African Americans were fed a high-fibre, low-fat African-style diet and rural Africans a high-fat, low-fibre western-style diet, under close supervision. In comparison with their usual diets, the food changes resulted in remarkable reciprocal changes in mucosal biomarkers of cancer risk and in aspects of the microbiota and metabolome known to affect cancer risk, best illustrated by increased saccharolytic fermentation and butyrogenesis, and suppressed secondary bile acid synthesis in the African Americans.

Notes

HITChip data matrix
A csv file containing HITChip phylogenetic microarray data matrix for the study samples. The data provides the absolute HITChip phylogenetic microarray signal estimate for 130 genus-like groups, preprocessed as described in the main article.
HITChip.csv
Metadata
CSV file containing the sample metadata in the HITChip data matrix. The variables are rounded or aggregated to ensure subject anonymity. 'NA' refers to missing values. The variable units and other information are as follows: - SampleID: unique sample identified corresponding to samples in the HITChip data matrix - subject: Subject identifier (some subjects have multiple time points) - bmi: Standard body-mass classification (underweight: <18.5; lean: 18.5-25; overweight: 25-30; obese: 30-35; severe obese: 35-40; morbid obese 40-45; superobese >45). - sex (male/female) - nationality: African American (AAM); Native African (AFR) - timepoint.group: Time point (1/2) within the group (ED/HE/DI) - timepoint.total: Time point in the overall data set (ED1 - HE1 -HE2 - DI1 - DI2 - ED2 ie. 1- 2- 3- 4- 5- 6) - group: Sample treatment group: as described in the main article; Dietary intervention (DI) / Home environment (HE) / Solid stool pre-colonoscopy (ED)
NMR data
JKNCRC_JLI121127_Okeefe.zip

Files

HITChip.csv

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Additional details

Related works

Is cited by
10.1038/ncomms7342 (DOI)