Biotechnologically-Produced Myconoside and Calceolarioside E Induce Nrf2 Expression in Neutrophils
Creators
- 1. Center of Plant Systems Biology and Biotechnology, 4000 Plovdiv, Bulgaria; amirova@cpsbb.eu (K.M.A.); andrey.marchev@yahoo.com (A.S.M.); Laboratory of Metabolomics, Department of Biotechnology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria
- 2. Laboratory of Experimental Immunotherapy, Department of Immunology, The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, 26 Georgi Bonchev Str., 1113 Sofia, Bulgaria;
- 3. Institute of Organic Chemistry with Center of Phytochemistry, Bulgarian Academy of Sciences, 9 Georgi Bonchev Str., 1113 Sofia, Bulgaria;
- 4. Kalina I. Alipieva
Description
The pathological manifestation of various diseases can be suppressed by the activation of
nuclear factor erythroid 2 p45-related factor 2 (Nrf2), a transcriptional regulator of the cellular redox
balance. Haberlea rhodopensis Friv. is a resurrection plant species endemic for Bulgaria, containing
biologically active phenylethanoid glycosides that might possess antioxidant or redox activity. This
study aimed to analyze the metabolic profile of in vitro cultured H. rhodopensis and to identify molecules
that increase Nrf2 expression in bone marrow neutrophils. Fractions B, D, and E containing
myconoside, or myconoside and calceolarioside E in ratios 1:0.6 and 0.25:1 were found to be the
most active ones. Fraction B (200 μg/mL) improved neutrophil survival and strongly increased the
Nrf2 intracellular level, while D and E, as well as, myconoside and calceolarioside E at the same
ratios had a superior effect. Calceolarioside E (32 μg/mL) had stronger activity than myconoside,
the effect of which was very similar to that of 2-cyano-3,12-dioxo-oleana-1,9(11)-dien-28-oic acid
methyl ester (CDDO-Me), used as a positive control. These data indicate that both molecules, used
alone or in combination have stimulatory activity on the endogenous Nrf2 level, indicating their
therapeutic potential to regulate the cellular redox homeostasis oxidative stress-associated pathologies.
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21. Amirova et al. 2021.pdf
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Additional details
Funding
- RESIST – Resurrection plants reveal secrets of vegetative desiccation tolerance 823746
- European Commission
- PlantaSYST – Establishment of a Center of Plant Systems Biology and Biotechnology for the translation of fundamental research into sustainable bio-based technologies in Bulgaria 739582
- European Commission
- Horizon 2020, Marie Skłodowska-Curie Actions, project RESIST - Resurrection plants reveal secrets of vegetative desiccation tolerance, grant (GA no. 823746) 823746
- European Commission