Hladiny lipoperoxidov v slinách a v plazme pri syndróme obštrukčného spánkového apnoe
Creators
- 1. Ústav lekárskej chémie, biochémie a klinickej biochémie, LF UK, Bratislava
- 2. Neurologická klinika SZU a UNB, Bratislava
- 3. I. neurologická klinika LF UK a UNB, Bratislava
Description
SÚHRN
Syndróm obštrukčného spánkového apnoe (OSA) je charakterizovaný opakovanou hypoxiou a následnou reoxygenáciou počas spánku, čo vedie ku vzniku oxidačného stresu a následnej peroxidácii lipidov. Predložená práca stanovuje hladiny biomarkera peroxidácie lipidov u pacientov s OSA a u kontrol a porovnáva tieto hladiny zistené v plazme a v slinách s cieľom nájsť alternatívne, neinvazívne médium na sledovanie oxidačného stresu. Zistili sme signifikantne zvýšené hladiny lipidových parametrov v plazme (okrem HDL) u dobrovoľníkov s OSA oproti kontrolám, ako aj hladín lipoperoxidov v slinách aj v plazme, ktoré navzájom vykazovali signifikantnú koreláciu.
ABSTRACT
Obstructive sleep apnea syndrome (OSA) is characterized by repeated episodes of hypoxia and subsequent reoxygenation during sleep, leading to oxidative stress and lipid peroxidation. The present work determines levels of the lipid peroxidation biomarker in patients with OSA and in controls and correlates these levels found in plasma and saliva in order to find an alternative, non-invasive medium for monitoring oxidative stress. We found significantly elevated plasma lipid parameters (excluding HDL) in OSA volunteers compared to controls, as well as salivary and plasma lipoperoxide levels, which showed a significant correlation. Our results suggest the existence of increased oxidative stress in individuals with OSA as well as impaired lipid metabolism. The significant correlation between lipoperoxides in plasma and saliva suggests that in addition to plasma also saliva can be used as a medium for determination of markers of oxidative stress.
Files
Laboratórna Diagnostika XXVI_1_2021 63–67.pdf
Files
(346.3 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:aa1734f3f92b14c3e1f822119deda6f5
|
346.3 kB | Preview Download |
Additional details
References
- Alzoghaibi, M. A. and BaHammam, A. S. O. (2005): Lipid peroxides, superoxide dismutase and circulating IL-8 and GCP-2 in patients with severe obstructive sleep apnea: A pilot study. Sleep and Breathing. doi: 10.1007/s11325-005-0022-1.
- Chiappin, S. et al. (2007): Saliva specimen: A new laboratory tool for diagnostic and basic investigation. Clinica Chimica Acta. doi: 10.1016/j.cca.2007.04.011.
- El-Saadani, M. et al. (1989): A spectrophotometric assay for lipid peroxides in serum lipoproteins using a commercially available reagent. Journal of Lipid Research. doi: 10.1016/ s0022-2275(20)38354-1
- Fernandez-Botran, R. et al. (2011): Correlations among inflammatory markers in plasma, saliva and oral mucosal transudate in post-menopausal women with past intimate partner violence. Brain, Behavior, and Immunity. doi: 10.1016/j. bbi.2010.09.023.
- Fiedorczuk, P., Stróżyński, A., Olszewska, E. (2020): Is the Oxidative Stress in Obstructive Sleep Apnea Associated with Cardiovascular Complications?—Systematic Review. Journal of Clinical Medicine. doi: 10.3390/jcm9113734.
- Lavie, L., Vishnevsky, A., Lavie, P. (2004): Evidence for lipid peroxidation in obstructive sleep apnea. Sleep. doi: 10.1093/ sleep/27.1.123.
- Minetto, M. et al. (2005): Differential responses of serum and salivary interleukin-6 to acute strenuous exercise. European Journal of Applied Physiology. doi: 10.1007/s00421-004- 1241-z.
- Passali, D. et al. (2015): Oxidative stress in patients with obstructive sleep apnoea syndrome. Acta otorhinolaryngologica Italica: organo ufficiale della Societa italiana di otorinolaringologia e chirurgia cervico-facciale. doi: 10.14639/0392-100X-895.
- Šiarnik, P. et al. (2020): Association of Lipid Profile with Sleep-Disordered Breathing in Patients with Acute Ischemic Stroke. Journal of Stroke Medicine. doi: 10.1177/25166085 20919211.
- Tomori, Z., Redhammer, R., Donič, V. (1999): Základy spánkovej medicíny. Košice: Vojenská letecká akadémia gen. Milana Rastislava Štefánika.
- Yardim-Akaydin, S. et al. (2013): Lipid peroxidation and DNA damage in apnea patients with or without metabolic syndrome. Sleep and Biological Rhythms. doi: 10.1111/sbr. 12012.