A novel seed plants gene regulates oxidative stress tolerance in Arabidopsis thaliana
Creators
- 1. BioAtlantis Ltd, Clash Industrial Estate, Tralee, Co. Kerry V92 RWV5, Ireland
- 2. Center of Plant Systems Biology and Biotechnology, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria
- 3. Institute of Biochemistry and Biology, University of Potsdam, Karl Liebknecht Str., 24-25, 14476 Potsdam-Golm, Germany
- 4. Department of Plant Breeding & Genetics, Faculty of Agricultural Sciences & Technology, Bahauddin Zakariya University, Bosan Road, Multan, 60800 Punjab, Pakistan
- 5. Max Planck Institute of Molecular Plant Physiology, Am Mühlenberg 1, 14476 Potsdam-Golm, Germany
- 6. Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria; Max‐Planck‐Institut für Molekulare Pflanzenphysiologie, Potsdam‐Golm, Germany
- 7. Center of Plant Systems Biology and Biotechnology, Plovdiv, Bulgaria; Max‐Planck‐Institut für Molekulare Pflanzenphysiologie, Potsdam‐Golm, Germany; Institute of Biochemistry and Biology, University of Potsdam, Karl Liebknecht Str., 24-25, 14476 Potsdam-Golm, Germany
- 8. Department of Molecular Pharmacology, University of Groningen, Antonius Deusinglaan 1, 9713 AV Groningen, The Netherlan
- 9. Center of Plant Systems Biology and Biotechnology, 139 Ruski Blvd, 4000 Plovdiv, Bulgaria; Department of Plant Physiology and Molecular Biology, University of Plovdiv, 24 Tsar Assen Str, 4000 Plovdiv, Bulgaria
Description
Oxidative stress can lead to plant growth retardation, yield loss, and death. The atr7 mutant of Arabidopsis thaliana exhibits pronounced tolerance to oxidative stress. Using positional cloning, confirmed by knockout and RNA interference (RNAi) lines, we identified the atr7 mutation and revealed that ATR7 is a previously uncharacterized gene with orthologs in other seed plants but with no homology to genes in lower plants, fungi or animals. Expression of ATR7-GFP fusion shows that ATR7 is a nuclear-localized protein. RNA-seq analysis reveals that transcript levels of genes encoding abiotic- and oxidative stress-related transcription factors (DREB19, HSFA2, ZAT10), chromatin remodelers (CHR34), and unknown or unchar-acterized proteins (AT5G59390, AT1G30170, AT1G21520) are elevated in atr7. This indicates that atr7 is primed for an upcoming oxidative stress via pathways involving genes of unknown functions. Collectively, the data reveal ATR7 as a novel seed plants-specific nuclear regulator of oxidative stress response
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A novel seed plants gene regulates oxidative stress tolerance in Arabidopsis thaliana.pdf
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