SUMCASTEC_180610_Discrimination of Glioblastoma Cancer Stem Cells by their UHF-Dielectrophoresis Crossover Frequency

We are introducing here firsts results of glioblastoma cell lines characterization; measuring their crossover frequencies by
dielectrophoresis (DEP) technics in the UHF frequency range (above 50 MHz). LN18 cells were cultured following different conditions, in order
to achieve an enrichment of cancer stem cells (CSCs). The DEP electrokinetic method is used to discriminate the CSCs from the differentiated
cells. In this study, microfluidic lab-on-chip systems implemented on Bipolar-Complementary Oxide Semiconductor (BiCMOS) technology is
used allowing single cell handling and analysis. Based on measurements of their own intracellular specificities, the enriched CSCs population,
cultured in dedicated define medium, have shown clear differences of DEP crossover frequency signatures compared to differentiated cells
cultured in normal medium. That demonstrates the concept and validates the technique efficiency for CSC discrimination in glioblastoma.