The Network of European Bioimage Analysts
Published March 8, 2021 | Version v1
Dataset Open

FRC-QE: A robust and comparable 3D microscopy image quality metric for cleared organoids - image data

Creators

  • 1. erlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany
  • 2. Molecular Oncology Center, Hospital Sirio-Libanese, São Paulo, SP, Brazil
  • 3. Charité - Universitätsmedizin Berlin, Dept. of Experimental Neurology, Center for Stroke Research Berlin, Berlin, Germany
  • 4. Berlin Institute of Health at Charité - Universitätsmedizin Berlin, Stem Cell Core Facility, Berlin, Germany
  • 5. Charité - Universitätsmedizin Berlin, Dept. of Experimental Neurology, Center for Stroke Research Berlin, Berlin, Germany / Charité - Universitätsmedizin Berlin, Dept. of Neurology, Berlin, Germany / Berlin Institute of Health at Charité –Universitätsmedizin Berlin, BIH Academy, Berlin, Germany
  • 6. Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine in the Helmholtz Association (MDC), Berlin, Germany and Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA, USA

Description

The ZIP file contains the image datasets that we acquired for benchmarking the FRC-QE algorithm. Data are ordered by microscope type and used clearing protocol.

For experimental procedure, acquisition settings and more details see:

FRC-QE: A robust and comparable 3D microscopy image quality metric for cleared organoids

Friedrich Preusser*, Natália dos Santos*, Jörg Contzen, Harald Stachelscheid, Érico Tosoni Costa, Philipp Mergenthaler#, Stephan Preibisch#

Bioinformatics, DOI: 10.1093/bioinformatics/btab160

FRC-QE is available on GitHub: GitHub - PreibischLab/FRC-QE

Funding: This work was supported by the Bundesministerium für Bildung und Forschung, Germany (BMBF, grant no. 031L0142A to S.P. and P.M., and in part by grants no. 16GW0191 and NUM-COVID 19 - Organo-Strat 01KX2021 to P.M. and H.S.) and HFSP grant RGP0021/2018-102 to S.P. and F.P. F.P. was funded by a PhD fellowship from Studienstiftung des deutschen Volkes. S.P. was funded by MDC Berlin and HHMI Janelia. N.S. was funded by FAPESP’s program of Research Internships Abroad (BEPE, 2019/06305-4) during her visit to the Preibisch lab at the MDC Berlin. P.M. was supported by the BIH-Charité Clinical Scientist Program funded by Charité – Universitätsmedizin Berlin and the Berlin Institute of Health. E.T.C. was funded by the Hospital Sirio-Libanese/HSL/SBSHSL. Establishment of the Charité High Content Shared Facility was supported by the Deutsche Forschungsgemeinschaft (DFG, grant no. INST 335/591-1).

Files

FRC-QE_example_data.zip

Files (12.1 GB)

Name Size Download all
md5:1fd2e2c0a0dc59b3fea4d2daecd52d7a
12.1 GB Preview Download