Study of reduction of antioxidant protection in the pathogenesis of experimental diabetic retinopathy

Sirman Ya. V., Preys N. I., Savytskyi I. V. Study of reduction of antioxidant protection in the pathogenesis of experimental diabetic retinopathy. Journal of Education, Health and Sport. 2020;10(11):306-311. eISSN 2391-8306. DOI http://dx.doi.org/10.12775/JEHS.2020.10.11.031

https://apcz.umk.pl/czasopisma/index.php/JEHS/article/view/JEHS.2020.10.11.031

https://zenodo.org/record/4549564

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Received: 16.10.2020. Revised: 30.10.2020. Accepted: 30.11.2020.

UDC: 616-06: 616-092.9

STUDY OF REDUCTION OF ANTIOXIDANT PROTECTION IN THE PATHOGENESIS OF EXPERIMENTAL DIABETIC RETINOPATHY

Ya. V. Sirman, N. I. Preys, I. V. Savytskyi

SE "Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine"

Sirman Yana Vadymivna, Candidate of Medical Sciences, Senior Researcher, Laboratory of Occupational Pathology,  State Enterprise "Ukrainian Research Institute of Transport Medicine of the Ministry of Health of Ukraine"

Preys Natalia Ihorivna, Candidate of Medical Sciences, Senior Researcher, Laboratory of Occupational Pathology,  SE "Ukrainian Scientific Research Institute of Transport Medicine Ministry of Health of Ukraine"

Savytskyi Ivan Volodymyrovych, Doctor of Medical Sciences, Professor, Head of the Department of Biomedical Sciences Odessa International Medical University

Author for correspondence: Savytskyi Ivan Volodymyrovych. 65039, Odessa, Fontanskaya road 4th, 29, tel. + 38050-381-21-83, e-mail farmakod@ukr.net

Abstract

The prevalence of diabetes mellitus (DM) is growing among the population of countries of the world, depending on the region, the level of economic development of the country, gender and age. The antioxidant system plays an important role in protecting the cells of the body from permanent and numerous damage that occurs due to the pathological influence of free radicals. Antioxidant enzymes, and peroxidases in particular, block the initiation of chain oxidation processes, preventing, among other things, the processes of DNA damage by free radicals that provoke the process of mutagenesis.

Our results indicate the development of oxidative stress in the pathogenesis of experimental diabetic retinopathy, as evidenced by a pronounced decrease in the activity of the antioxidant defense enzyme under the conditions of our study.

Already on the thirtieth day of the development of experimental diabetic retinopathy, a decrease in peroxidase activity was observed, followed by the progression of pathological changes on the sixtieth day of the study.

The most pronounced decrease in the activity of the studied marker of antioxidant defense was found at the 3rd stage of the experiment.

Key words: diabetic retinopathy; diabetes mellitus; experimental model; oxidative stress; peroxidase