Published April 22, 2019 | Version v1
Preprint Open

Systematics of 'lithistid' tetractinellid demosponges from the Tropical Western Atlantic – implications for phylodiversity and bathymetric distribution

  • 1. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Munich, Germany; Department of Biology, NordCEE (Current address), Southern University of Denmark, Odense, Denmark
  • 2. Harbor Branch Oceanographic Institute, Florida Atlantic University, Ft Pierce, Florida, USA
  • 3. Institute of Paleobiology, Polish Academy of Sciences, Warszawa, Poland
  • 4. Department of Medicinal Chemistry, Uppsala University, Uppsala, Sweden
  • 5. National Centre for Coasts and Oceans, National Institute of Water and Atmospheric Research, Newmarket, Auckland, New Zealand
  • 6. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Munich, Germany; GeoBio-Center, Ludwig-Maximilians-Universität München, Munich, Germany; SNSB-Bayerische Staatssammlung für Paläontologie und Geologie, Munich, Germany
  • 7. Department of Earth and Environmental Sciences, Ludwig-Maximilians-Universität München, Munich, Germany; GeoBio-Center, Ludwig-Maximilians-Universität München, Munich, Germany

Description

ABSTRACT

Background: Among all present demosponges, lithistids represent a polyphyletic group with exceptionally well preserved fossils dating back to the Cambrian. Knowledge of their recent diversity, particularly in the Tropical Western Atlantic Ocean (TWA) where they are common in deep waters, is scarce making any comparison between present and past major ‘lithistid’ faunas difficult. In addition, the lack of sufficient molecular and morphological data hamper any predictions on phylogenetic relationships or phylodiversity from this region. The Harbor Branch Oceanographic Institute (HBOI, Fort Pierce, Florida) holds the largest collection of TWA lithistid sponges worldwide, however, the majority remain to be taxonomically identified and revised.

Methods/Principal Findings: In this study we provide sequences of 249 lithistid demosponges using two independent molecular markers (28S rDNA (C1-D2) and cox1 mtDNA). In addition, a morphological documentation of 70 lithistid specimens is provided in the database of the Sponge Barcoding Project (SBP). This integrated dataset represents the largest and most comprehensive of the TWA lithistids to date. The phylogenetic diversity of ‘lithistid’ demosponges in the Bahamas and Jamaica are high in comparison to other TWA regions; Theonellidae and Corallistidae dominate the fauna, while Neopeltidae and Macandrewiidae are rare. A new tetractinellid suborder, one new genus and several new species are recognized and the Pacific ‘lithistid’ genera, Herengeria and Awhiowhio, are reported from the TWA for the first time. The higher-taxa relationships of desma-bearing tetractinellids are discussed and topics for revision suggested.

Conclusion: This first integrative approach of TWA ‘lithistid’ demosponges contributes to a better understanding of their phylogenetic affinities, diversity and bathymetric distribution patterns within the TWA. As in the Pacific, the TWA ‘lithistid’ demosponges dominate deep-water habitats. Deeper taxonomic investigations will undoubtedly contribute to a better comparison between present major ‘lithistid’ faunas and their fossil record in the Mesozoic.

Notes

ACKNOWLEDGMENTS We acknowledge the Government of the Bahamas, Curac ̧ao, Jamaica, Turks and Caicos, Bonaire and St. Vincent Islands, Martinique, Guadeloupe, Puerto Rico, Cuba, Jamaica, Honduras, Gulf of Mexico, and the Philippines, for granting permission to conduct research in their territorial waters. Thirty-two new specimens and associated data were supplied by the NIWA Invertebrate Collection (NIC) for sequencing; we are particularly grateful to Sadie Mills for her diligent assistance with loans. A further 31 specimens and associated images and data were supplied by Lori J. Bell and the Coral Reef Research Foundation (CRRF) for sequencing; these were collected under contract to the U.S. National Cancer Institute. We thank colleagues Amy Wright, John Reed and Megan Conkling (HBOI-FAU) for their assistance with the HBOI collection of samples and associated data. AS thanks Sergio Vargas (Dept. of Earth- and Environmental Sciences, LMU Munich, Germany) for his help with the modification of the phylodiversity script. Nicole Enghuber is thanked for her help in spicule preparations and Simone Scha ̈tzle is thanked for sequencing assistance (both Dept. of Earth- and Environmental Sciences, LMU Munich, Germany). AUTHOR CONTRIBUTIONS Astrid Schuster conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft, identified genera/species. Shirley A Pomponi contributed reagents/materials/analysis tools, approved the final draft, identified genera/species and contributed to the sampling. Andrzej Pisera contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, identified genera/species. Paco Cárdenas authored or reviewed drafts of the paper, approved the final draft, identified genera/species. Michelle Kelly contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft, identified genera/species and contributed to the sampling. Gert Wörheide contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft. Dirk Erpenbeck conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft. FUNDING Financial support for this study was provided by the German Science Foundation to DE and GW (DFG ER 611/3-1, DFG Wo869/15-1, respectively). The LMU Mentoring and the HELGE AX:Son JOHNSON STIFTELSE provided funding for AS to visit HBOI (Florida, USA) and NIWA (National Institute of Water and Atmospheric Research, Auckland and Wellington in New Zealand). Financial support for the R/V Seward Johnson/Johnson Sea Link I+II expedition was provided by HBOI. MK's participation was funded by NIWA under Coasts and Oceans Research Programme 2 Marine Biological Resources: Discovery and definition of the marine biota of New Zealand (2016/2017 and 2017/2018 SCI). PC and SP received support from the European Union's Horizon 2020 research and innovation program through the SponGES project (Grant agreement No. 679849). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Additional details

Funding

SponGES – Deep-sea Sponge Grounds Ecosystems of the North Atlantic: an integrated approach towards their preservation and sustainable exploitation 679849
European Commission