Desulfovibrio vulgaris Hildenborough Genetic Constructs
Creators
- 1. University of Missouri
- 2. Lawrence Berkeley National Laboratory
- 3. Bayer (United States)
- 4. IBM Research – Thomas J. Watson Research Center
- 5. University of Oklahoma
- 6. William Woods University
- 7. Southern Illinois University Carbondale
Description
The dissimilatory sulfate-reducing Deltaproteobacterium, Desulfovibrio vulgaris Hildenborough (ATCC 29579), was chosen by the LBNL-led research collaboration ENIGMA to explore tools and protocols for bringing this anaerobe to model status. Here we describe a collection of genetic constructs generated by ENIGMA, which are available to the research community.
Notes
A ReadMe file is being uploaded which is associated with the lists of constructs and provides more detailed descriptions of and publications for the protocols used.
Prior to pooling a specialized subset of transposon mutants to determine gene fitness data, all members should be streaked to determine the lack of aerobic contaminants. A pool of the ca. 11,000 mutants isolated and sequenced was found to have contamination. Of the mutants we have examined, only one or two showed contamination. Often a second transposon mutant interrupting the same gene (usually a different site) is part of the collection that may show no contamination.
Select publications from this work are noted in the ReadMe file and in the Excel file "Genetic Constructs of Desulfovibrio vulgaris Hildenborough final" in sheet 5, "Boutique Deletions," column F.
Funding provided by: U.S. Department of Energy
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000015
Award Number: DE-AC02-05CH11231
Funding provided by: University of Missouri
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100007165
Award Number: Mizzou Advantage to KD
Funding provided by: U.S. Department of Energy
Crossref Funder Registry ID: http://dx.doi.org/10.13039/100000015
Award Number: DE-FG02-08ER64691
Files
Desulfovibrio_vulgaris_constructs.zip
Files
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