Published July 27, 2015
| Version v1
Journal article
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Laser-scanning cytometry can quantify human adipocyte browning and proves effectiveness of irisin
- 1. University of Debrecen
Description
Laser-scanning cytometry is presented as a tool allowing population scale analysis of ex vivo human brown adipogenic differentiation. It combines texture analysis and detection of Ucp1 protein content in single brown adipocytes of mixed cell populations with gene expression pattern and functional characteristics of browning. Using this method we could validate mouse data in human samples demonstrating the effectiveness of irisin to induce “beige” differentiation of subcutaneous white adipocytes.
Notes
EUR 1,165 APC fee funded by the EC FP7 Post-Grant Open Access Pilot
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Fesus_srep12540-s1_TRANSCOM_FP7.pdf
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