Published January 20, 2021 | Version V1.0
Technical note Open

Cryosectioning of fixed and frozen tissue

  • 1. MNI, McGill University

Description

This protocol will provide a standardized procedure on methods developed for cryosectioning of fixed or fresh frozen tissue in Optimal Cutting Temperature (OCT) using a motorized cryostat. This SOP also outlines minimum assessment criteria that should be in place to evaluate the quality and integrity of frozen tissue sections and troubleshooting steps when issues with sectioning arise.

Files

EDDU-017-01_Cryosectioning of frozen tissue_v1.0_merged.pdf

Files (1.0 MB)