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Published December 9, 2020 | Version v1.5
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Minimal genome-wide human CRISPR-Cas9 library


  • 1. Sanger Institute


Minimal genome-wide human CRISPR-Cas9 library release

CRISPR guide-RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.



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