IRCCS Humanitas Research Hospital & Humanitas University
Published December 9, 2020 | Version v1
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Dataset related to article "Macrophage morphology of tumor-associated macrophages as a correlate of metabolism with prognostic significance in colorectal liver metastases"

Authors/Creators

  • 1. Humanitas Clinical and Research Center-IRCCS and Humanitas University
  • 2. Humanitas Clinical and Research Center-IRCCS
  • 3. Humanitas Clinical and Research Center-IRCCS and University of Milan
  • 4. Humanitas Clinical and Research Center-IRCCS and Humanitas University

Description

This record contains data related to article "Morphology of tumor-associated macrophages as a correlate of metabolism with prognostic significance in colorectal liver metastases"

Abstract

It has long been known that in vitro polarized macrophages differ in morphology. Stemming from a conventional immunohistology observation, we set out to test the hypothesis that morphology of tumor-associated macrophages (TAMs) in colorectal liver metastasis (CLM) represents a correlate of functional diversity with prognostic significance. Density and morphological metrics of TAMs were measured and correlated with clinicopathological variables. While density of TAMs did not correlate with survival of CLM patients, the cell area identified small (S-TAM) and large (L-TAM) macrophages that were associated with 5-yr disease-free survival rates of 27.8% and 0.2%, respectively (P < 0.0001). RNA sequencing of morphologically distinct macrophages identified LXR/RXR as the most enriched pathway in large macrophages, with upregulation of genes involved in cholesterol metabolism, scavenger receptors, MERTK, and complement. In single-cell analysis of mononuclear phagocytes from CLM tissues, S-TAM and L-TAM signatures were differentially enriched in individual clusters. These results suggest that morphometric characterization can serve as a simple readout of TAM diversity with strong prognostic significance.

Notes

This record contains data related to the article "Morphology of tumor-associated macrophages as a correlate of metabolism with prognostic significance in colorectal liver metastases" funded by Associazione Italiana per la Ricerca sul Cancro grant number AIRC 5x1000 IG-21147

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Additional details

Related works

Is supplement to
32785653 (PMID)
10.1084/jem.20191847 (DOI)