RNAseq2020
Description
Fastq files and sample info list from RNAseq experiment (November 2020) on tomato moneymaker wildtype and mutants lines for a TF involved in glandular trichome development and specialised metabolism.
Plant material: second par of corresponding leaflets on the 4th leaf of 4 weeks old plant; whole stems and petioles. Harvested on 23/07/2020. Snap-frozen in N2 and stored at -80 C. Frozen leaves were grounded and omogenized. Frozen stems and petioles were vortex and trichomes shaken off.
RNA extraction: Qiagen RNeasy Plant Mini Kit (50) (Cat No./ID: 74904) for RNA isolation and purification and Qiagen RNase-Free DNase Set (50) (Cat No./ID: 79254) for on-columd DNAse treatment.
Samples: 60x, see sample info for details.
2x Illumina High Output v2.5 kit 75 cycles, ~13Mreads per sample.
Files
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