Published November 30, 2020 | Version v1
Journal article Open

STUDY OF ANTI-INFLAMMATORY EFFECT OF CONCENTRATED JUICE AND GRANULES FROM VACCINIUM OXYCOCCOS FRUITS ON THE MODEL OF BACTERIAL CYSTITIS IN RATS

Creators

  • 1. Ivano-Frankivsk National Medical University

Description

The fruits of marsh cranberries due to the content of phenolic compounds, in particular proanthocyanidins, are effective in the prevention and treatment of cystitis [1].

The aim of the research was to study the anti-inflammatory properties of condensed juice of Vaccinium oxycoccos fruits and granules “Cranberry” in a model of acute bacterial cystitis in rats.

Materials and methods. The anti-inflammatory properties of condensed juice from Vaccinium oxycoccos fruits and granules “Cranberry” were studied in a model of acute bacterial cystitis in rats, which was caused by three daily transurethral injection into the bladder of 0.3 ml of E. coli strain at a concentration of 106 CFU/ml. “Canefron® H” was used as a reference drug. Tissues were studied histologically by assessing the structure of the bladder wall and the condition of blood vessels.

Results. Infection of animals with E. coli resulted in a significant increase in the desquamation of the transitional epithelium, edematous changes and narrowing of blood vessels in the tissues of the bladder wall. During treatment with the drug “Canefron® N” there was a decrease in cell infiltration and desquamation of cells, dilation of blood vessels. When animals used condensed juice from Vaccinium oxycoccos fruits and granules “Cranberry” in the histological picture of the walls of the bladder there was a decrease in desquamation, edema and cellular infiltration, the lumen of blood vessels was approaching normal.

Conclusions. The use of condensed juice from Vaccinium oxycoccos fruits and granules “Cranberry” was accompanied by a positive effect on the tissues of the walls and blood vessels of the bladder of rats (there was a decrease in desquamation of the superficial layers of the transitional epithelium and normalization of the histological picture of vascular entanglement, that was not inferior to the comparison drug “Canefron® N”.

Files

STUDY OF ANTI-INFLAMMATORY EFFECT OF CONCENTRATED JUICE AND GRANULES FROM VACCINIUM OXYCOCCOS FRUITS ON THE MODEL OF BACTERIAL CYSTITIS IN RATS.pdf

Files (2.7 MB)

Additional details

References

  • Liutikova, M. N., Botirov, E. Kh. (2015). Khimicheskii sostav i prakticheskoe primenenie iagod brusniki i kliukvy. Khimiia rastitelnogo syria, 2, 5–27. Available at: https://cyberleninka.ru/article/n/himicheskiy-sostav-i-prakticheskoe-primenenie-yagod-brusniki-i-klyukvy/viewer
  • Unifikovanyi klinichnyi protokol medychnoi dopomohy. Diahnostyka ta likuvannia infektsii sechovydilnoi systemy (2011). Available at: https://www.dec.gov.ua/wp-content/uploads/2019/11/816dod1_2.pdf
  • Yatsiuk, K. M., Feodorovska, M. I., Kutsyk, R. V. (2018). The investigation of the cranberry (Vaccinium oxycoccos L.) concentrated juice antimicrobial activity. Farmatsevtychnyi Zhurnal, 1, 84–92. doi: http://doi.org/10.32352/0367-3057.1.17.11
  • Davidson, E., Zimmermann, B. F., Jungfer, E., Chrubasik-Hausmann, S. (2013). Prevention of Urinary Tract Infections withVacciniumProducts. Phytotherapy Research, 28 (3), 465–470. doi: http://doi.org/10.1002/ptr.5047
  • LaPlante, K. L., Sarkisian, S. A., Woodmansee, S., Rowley, D. C., Seeram, N. P. (2012). Effects of Cranberry Extracts on Growth and Biofilm Production of Escherichia coli and Staphylococcus species. Phytotherapy Research, 26 (9), 1371–1374. doi: http://doi.org/10.1002/ptr.4592
  • Kirillov, V. I., Bogdanova, N. A., Morozov, S. L. (2018). Non-antibacterial alternative measures for urinary tract infection in children. Part 1. Suppression of the microbial initiation of the inflammation. Rossiyskiy Vestnik Perinatologii i Pediatrii (Russian Bulletin of Perinatology and Pediatrics), 63 (1), 106–112. doi: http://doi.org/10.21508/1027-4065-2018-63-1-106-112
  • Ulrey, R. K., Barksdale, S. M., Zhou, W., van Hoek, M. L. (2014). Cranberry proanthocyanidins have anti-biofilm properties against Pseudomonas aeruginosa. BMC Complementary and Alternative Medicine, 14 (1), 414–499. doi: http://doi.org/10.1186/1472-6882-14-499
  • Gupta, K., Chou, M. Y., Howell, A., Wobbe, C., Grady, R., Stapleton, A. E. (2007). Cranberry Products Inhibit Adherence of P-Fimbriated Escherichia Coli to Primary Cultured Bladder and Vaginal Epithelial Cells. Journal of Urology, 177 (6), 2357–2360. doi: http://doi.org/10.1016/j.juro.2007.01.114
  • Rane, H. S., Bernardo, S. M., Howell, A. B., Lee, S. A. (2013). Cranberry-derived proanthocyanidins prevent formation of Candida albicans biofilms in artificial urine through biofilm- and adherence-specific mechanisms. Journal of Antimicrobial Chemotherapy, 69 (2), 428–436. doi: http://doi.org/10.1093/jac/dkt398
  • LaPlante, K. L., Sarkisian, S. A., Woodmansee, S., Rowley, D. C., Seeram, N. P. (2012). Effects of Cranberry Extracts on Growth and Biofilm Production of Escherichia coli and Staphylococcus species. Phytotherapy Research, 26 (9), 1371–1374. doi: http://doi.org/10.1002/ptr.4592
  • Unifikovanyi klinichnyi protokol medychnoi dopomohy. Hostryi neuskladnenyi tsystyt u zhinok (2011). Nakaz MOZ Ukrainy No. 816. 23.11.2011. Available at: http://search.ligazakon.ua/l_doc2.nsf/link1/MOZ23600.html
  • Hryhorenko, V. M., Romashchenko, O. V., Biloholovska, V. V., Volkov, S. S. (2016). Stratehiia likuvannia neuskladnenykh infektsii nyzhnikh sechovyvidnykh shliakhiv. Novosty medytsyni y farmatsyy v Ukrayne, 11 (585), 9–14. Available at: http://www.mif-ua.com/archive/article/43685
  • Nastanova Yevropeiskoi asotsiatsii urolohiv iz likuvannia infektsii sechovykh shliakhiv (EAU, 2012/2013). Available at: http://www.mif-ua.com/archive/article/36615
  • Medved, V. I., Islamova, E. V., Kirilchuk, M. E. (2015). Ekstrakt iagod kliukvy v profilaktike i lechenii infektsii mochevyvodiaschikh putei u beremennykh. Zdorove zhenschiny, 1 (97), 36–40.Available at: http://nbuv.gov.ua/UJRN/Zdzh_2015_1_8
  • Safronova, I. V., Goldina, I. A., Gaidul, K. V. (2015). Biologicheski aktivnye komponenty kliukvy i ikh primenenie v meditsine. Innovatsii i prodovolstvennaia bezopasnost, 1 (7), 6–18.
  • Yatsiuk, K. M., Fedorovska, M. I., Kovalska, N. P. (2015). Zhuravlyna bolotna (Oxycoccus palustris pers.L.) yak dzherelo dlia oderzhannia likarskykh zasobiv. Fitoterapiia chasopys, 2, 26–28.
  • Yatsyuk, K. M., Fedorovska, M. I., Barna, O. M. (2015). Granules composition and technology development based on condensed cranberry juice for urological use K. M. The Pharma Innovation Journal, 4 (8), 29–30. Available at: http://www.thepharmajournal.com/archives/?year=2015&vol=4&issue=8&ArticleId=662
  • Yatsiuk, K. M., Fedorovska, M. I. (2016). Pat. No. 116426 UA. Zasib dlia zastosuvannia v urolohii. MPK: A61K 9/20, A61K 36/00. No. u 201610598; declareted: 20.10.2016; published: 25.05.2017, Biul. No. 10, 4.
  • Yatsyuk, K. M., Fedorovskaya, M. I., Marchyshyn, S. M., Budniak, L. I., Slobodianiuk, L. V. (2019). Obtaining and standardization of condensed juice from cranberries marsh fruits. Medical and Clinical Chemistry, 2, 55–60. doi: http://doi.org/10.11603/mcch.2410-681x.2019.v.i2.10294
  • Directive 2010/63/EU Legislation for the protection of animals used for scientific purposes. Available at: http://ec.europa.eu/environment/chemicals/lab_animals/legislation_en.htm
  • ARRIVE guidelines. Available at: https://www.nc3rs.org.uk/arrive-guidelines
  • Kirpatovskiy, V. I., Mudraya, I. S., Grekov, E. A., Kabanova, I. V., Golovanov S.A., Drojjeva, V. V. et. al. (2013). Influence of the experimental metabolic syndrome on the function of the urinary bladder in rats. Ekspirimentalnaia i klinicheskaia urologiia, 1, 8–13. Available at: https://ecuro.ru/sites/default/files/ECU_8-13%20kirpatovskiy.pdf
  • Amdii, R. E., Al-Shukri, S. H., Kuzmin, I. V., Sorokin, N. V., Chaplitskii, E. A., Skvortsov, M. V. et. al. (2016). Use of Kanefron in treatment of acute uncomplicated cystitis in women. Urologicheskie Vedomosti, 6 (2), 16. doi: http://doi.org/10.17816/uroved6216-22
  • Davidson, E., Zimmermann, B. F., Jungfer, E., Chrubasik-Hausmann, S. (2013). Prevention of Urinary Tract Infections withVacciniumProducts. Phytotherapy Research, 28 (3), 465–470. doi: http://doi.org/10.1002/ptr.5047
  • Guay, D. R. P. (2009). Cranberry and Urinary Tract Infections. Drugs, 69 (7), 775–807. doi: http://doi.org/10.2165/00003495-200969070-00002
  • Howell, A. B. (2007). Bioactive compounds in cranberries and their role in prevention of urinary tract infections. Molecular Nutrition & Food Research, 51 (6), 732–737. doi: http://doi.org/10.1002/mnfr.200700038