SACCHARIFICATION OF IONIC LIQUID TREATED RICE STRAW

 Enzymatic saccharification of ionic liquid treated rice straw (IL-RS) was studied. Here, Box-Behnken based response surface methodology (BBD based RSM) was applied to find the suitable dosages of cellulase (Cellic® CTec2), xylanase (Cellic® HTec2) and Triton X-100 on hydrolysis of IL-RS. The result shows that cellulase and xylanase significantly affected (p-value < 0.05) the reducing sugar yields derived from the hydrolysis process of IL-RS. The experimental data obtained agreed with the predicted values (R2=0.98), and the data were fitted to second-order polynomial equation. The maximal sugar yield was obtained with loads of Cellic® CTec2 at 50 FPU/g substrate, Cellic® HTec2 at 100 XU/g substrate, and Triton X-100 at 0.375 g/g substrate, giving reducing sugar 3,828.86 μg/mL at 24-h incubation. Therefore, the model could be successfully used to identify the effective combinations of the three factors for increasing reducing sugar yield.