Genomic blueprints of sponge- prokaryote symbiosis are shared by low abundant and cultivatable Alphaproteobacteria
Creators
- 1. Faculty of Science and Technology, Algarve University, Gambelas, 8005-139, Faro, Portugal & centre of Marine Sciences, Algarve University, Gambelas, 8005-139, Faro, Portugal
- 2. institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico (IST), University of Lisbon, 1049-001, Lisbon, Portugal
- 3. RD3 Marine Microbiology, GEOMAR Helmholtz Centre for Ocean Research Kiel, 24105, Kiel, Germany
- 4. centre of Marine Sciences, Algarve University, Gambelas, 8005-139, Faro, Portugal
- 5. Department of environmental Microbiology, Helmholtz Centre for Environmental Research - UFZ, 04318, Leipzig, Germany
- 6. RD3 Marine Microbiology, GEOMAR Helmholtz Centre for Ocean Research Kiel, 24105, Kiel, Germany & Christian-Albrechts- Universität zu Kiel, 24118, Kiel, Germany
- 7. centre of Marine Sciences, Algarve University, Gambelas, 8005-139, Faro, Portugal & institute for Bioengineering and Biosciences (iBB), Instituto Superior Técnico (IST), University of Lisbon, 1049-001, Lisbon, Portugal
Description
ABSTRACT
Marine sponges are early-branching, filter-feeding metazoans that usually host complex microbiomes comprised of several, currently uncultivatable symbiotic lineages. Here, we use a low-carbon based strategy to cultivate low-abundance bacteria from Spongia officinalis. This approach favoured
the growth of Alphaproteobacteria strains in the genera Anderseniella, Erythrobacter, Labrenzia, Loktanella, Ruegeria, Sphingorhabdus, Tateyamaria and Pseudovibrio, besides two likely new genera in the Rhodobacteraceae family. Mapping of complete genomes against the metagenomes of S. officinalis, seawater, and sediments confirmed the rare status of all the above-mentioned lineages in the marine realm. Remarkably, this community of low-abundance Alphaproteobacteria possesses several genomic attributes common to dominant, presently uncultivatable sponge symbionts, potentially contributing to host fitness through detoxification mechanisms (e.g. heavy metal and metabolic waste removal, degradation of aromatic compounds), provision of essential vitamins (e.g. B6 and B12 biosynthesis), nutritional exchange (especially regarding the processing of organic sulphur and nitrogen) and chemical defence (e.g. polyketide and terpenoid biosynthesis). None of the studied taxa displayed signs of genome reduction, indicative of obligate mutualism. Instead, versatile nutrient metabolisms along with motility, chemotaxis, and tight-adherence capacities - also known to confer environmental hardiness – were inferred, underlying dual host-associated and free-living life strategies adopted by these diverse sponge-associated Alphaproteobacteria.
Notes
Files
Karimi et al 2019-s41598-019-38737-x.pdf
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Additional details
Related works
- Is derived from
- Journal article: https://www.nature.com/articles/s41598-019-38737-x.pdf (URL)
- Dataset: https://www.nature.com/articles/s41598-019-38737-x#Ack1 (URL)